Recombinant Human Junctional Sarcoplasmic Reticulum protein is a Human Full Length protein, in the 1 to 331 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S M T T R A W E E L D G G L G S C Q A L E D H S A L A E T Q E D R A S A T P R L A D S G S V P H D S Q V A E G P S V D T R P K K M E K E P A A R G T P G T G K E R L K A G A S P R S V P A R K K A Q T A P P L Q P P P P P P A L S E E L P W G D L S L N K C L V L A S L V A L L G S A F Q L C R D A V P G E A A L Q A R V P E P W V P P S S A P R E P S S P L P K F E A Q A P P S A P P A P R A E A E V R P K I P G S R E A A E N D E E E P G E A T G E A V R E D R V T L A D R G P K E R P R R E G K P R K E K P R K E E R P K K E R P R K E E R P R A A R E P R E A L P Q R W E S R E G G H R P W A R D S R D A E P R K K Q A W V S P R R P D E E Q R P G S R Q K L R A G K G R D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in skeletal muscle excitation/contraction coupling (EC), probably acting as a regulator of the voltage-sensitive calcium channel CACNA1S. EC is a physiological process whereby an electrical signal (depolarization of the plasma membrane) is converted into a chemical signal, a calcium gradient, by the opening of ryanodine receptor calcium release channels. May regulate CACNA1S membrane targeting and activity.
Alternative names
JP45, JSRP1, Junctional sarcoplasmic reticulum protein 1, Junctional-face membrane protein of 45 kDa homolog, JP-45
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Recombinant Human Junctional Sarcoplasmic Reticulum protein is a Human Full Length protein, in the 1 to 331 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96MG2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S M T T R A W E E L D G G L G S C Q A L E D H S A L A E T Q E D R A S A T P R L A D S G S V P H D S Q V A E G P S V D T R P K K M E K E P A A R G T P G T G K E R L K A G A S P R S V P A R K K A Q T A P P L Q P P P P P P A L S E E L P W G D L S L N K C L V L A S L V A L L G S A F Q L C R D A V P G E A A L Q A R V P E P W V P P S S A P R E P S S P L P K F E A Q A P P S A P P A P R A E A E V R P K I P G S R E A A E N D E E E P G E A T G E A V R E D R V T L A D R G P K E R P R R E G K P R K E K P R K E E R P K K E R P R K E E R P R A A R E P R E A L P Q R W E S R E G G H R P W A R D S R D A E P R K K Q A W V S P R R P D E E Q R P G S R Q K L R A G K G R D
- Accession
- Q96MG2
- Protein length
- Full Length
- Predicted molecular weight
- 38 kDa
- Amino acids
- 1 to 331
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab171720 was purified by anion-exchange and gel-filtration chromatography techniques.
General info
- Function
Involved in skeletal muscle excitation/contraction coupling (EC), probably acting as a regulator of the voltage-sensitive calcium channel CACNA1S. EC is a physiological process whereby an electrical signal (depolarization of the plasma membrane) is converted into a chemical signal, a calcium gradient, by the opening of ryanodine receptor calcium release channels. May regulate CACNA1S membrane targeting and activity.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The junctional sarcoplasmic reticulum (JSR) also known as the triad junction is a specialized region of the sarcoplasmic reticulum in muscle cells. It plays key role in excitation-contraction coupling. JSR contains proteins that facilitate the rapid release of calcium ions. It often mentioned in context of its interaction with the ryanodine receptor (RyR) and the dihydropyridine receptor (DHPR). The molecular weight of various JSR-associated proteins can differ widely with the ryanodine receptor weighing approximately 565 kDa. JSR is mostly found in skeletal and cardiac muscle tissues where it links with transverse tubules to create the triad structure essential for muscle function.
- Biological function summary
The JSR ensures efficient calcium signalling necessary for muscle contractions. This region acts as main calcium storage in muscle cells and releases calcium upon stimulation which is critical for muscle contraction. The JSR forms complex with other protein components such as the ryanodine receptor and calsequestrin that buffer calcium ions and regulate their release. Calcium ions rapidly diffuse from the sarcoplasmic reticulum into the cytoplasm to bind to the myofilaments triggering contraction.
- Pathways
Calcium release from the JSR influences muscle contraction and protocols including excitation-contraction coupling. It connects to the calcium signaling pathway and plays an important part in the contraction mechanism in both skeletal and cardiac muscle. The JSR involves important interactions with proteins like the dihydropyridine receptor located in the transverse tubule initiating calcium ion release from the ryanodine receptor. This release forms a link between electrical signals and mechanical contractions in muscle fibers.
- Associated diseases and disorders
Improper function or mutations affecting the JSR lead to conditions like malignant hyperthermia and cardiac arrhythmias. Malignant hyperthermia a genetic disorder is often triggered by mutations in the ryanodine receptor part of the JSR causing abnormal calcium release and resulting in deadly muscle rigidity and heat production. Cardiac arrhythmias can emerge from disruptions in calcium handling within the JSR particularly when irregular calcium releases affect normal heart rhythm. The dysregulation can involve interactions with proteins such as calsequestrin which plays role in maintaining proper calcium storage and release dynamics within the JSR.
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SDS-PAGE - Recombinant Human Junctional Sarcoplasmic Reticulum protein (ab171720)
15% SDS-PAGE analysis of ab171720 (3 μg).
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