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AB224785

Recombinant Human Kallikrein 5 protein (His tag)

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Recombinant Human Kallikrein 5 protein (His tag) is a Human Fragment protein, in the 67 to 293 aa range, expressed in Baculovirus infected insect cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

SCTE, UNQ570/PRO1132, KLK5, Kallikrein-5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme, KLK-L2

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SDS-PAGE - Recombinant Human Kallikrein 5 protein (His tag) (AB224785)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Kallikrein 5 protein (His tag) (AB224785)

15% SDS-PAGE analysis of 3 μg ab224785.

MW : 28-40 kDa (SDS-PAGE under reducing conditions).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y337

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ADLIINGSDCDMHTQPWQAALLLRPNQLYCGAVLVHPQWLLTAAHCRKKVFRVRLGHYSLSPVYESGQQMFQGVKSIPHPGYSHPGHSNDLMLIKLNRRIRPTKDVRPINVSSHCPSAGTKCLVSGWGTTKSPQVHFPKVLQCLNISVLSQKRCEDAYPRQIDDTMFCAGDKAGRDSCQGDSGGPVVCNGSLQGLVSWGDYPCARPNRPGVYTNLCKFTKWIQETIQANSHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"26.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":293,"aminoAcidStart":67,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"Q9Y337","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 5 also known as KLK5 or stratum corneum tryptic enzyme is a serine protease enzyme with a molecular weight of approximately 30 kDa. It is encoded by the KLK5 gene and is part of the kallikrein family. KLK5 is mainly expressed in the skin but also found in other tissues such as the esophagus and breast. The enzyme plays a mechanical role in skin barrier function by processing and activating other kallikreins catalyzing the degradation of corneodesmosomes which are essential for skin desquamation.
Biological function summary

KLK5 assists in maintaining the skin's outermost layer by regulating the desquamation process. It does not operate in isolation but is an intrinsic part of the kallikrein proteolytic cascade which involves several other kallikrein-related peptidases including KLK7 and KLK14. This cascade ensures proper turnover and shedding of damaged or mature cells from the skin surface playing an important role in skin homeostasis and renewal. It is significant in forming a protective barrier against environmental insults.

Pathways

KLK5 integrates into the skin barrier and inflammation regulation pathways. Its activity influences the activation of other proteases and signaling molecules involved in skin inflammation and repair processes. Related proteins like protease-activated receptors (PARs) interact with KLK5 mediating downstream signaling that impacts inflammation and tissue remodeling. The cascades involving KLK5 serve as checkpoints in cutaneous homeostasis and immune responses.

KLK5 has an important association with skin conditions like atopic dermatitis and Netherton syndrome. In atopic dermatitis abnormal KLK5 expression leads to compromised skin barrier integrity exacerbating the condition. It also relates to LEKTI (lympho-epithelial Kazal-type related inhibitor) dysfunction in Netherton syndrome where uncontrolled KLK5 activity contributes to severe barrier defects. The interactions between KLK5 and these disorders highlight its role in pathophysiological processes affecting skin health.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

May be involved in desquamation.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

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Product protocols

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Target data

May be involved in desquamation.
See full target information KLK5
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