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AB153781

Recombinant Human Kallistatin/PI-4 protein

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Recombinant Human Kallistatin/PI-4 protein is a Human Full Length protein, in the 21 to 427 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, HPLC.

View Alternative Names

KST, PI4, SERPINA4, Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, Serpin A4, PI-4

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P29622

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 8 Constituents: 0.88% Sodium chloride, 0.79% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Kallistatin.
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Sequence info

[{"sequence":"QLHVEHDGESCSNSSHQQILETGEGSPSLKIAPANADFAFRFYYLIASETPGKNIFFSPLSISAAYAMLSLGACSHSRSQILEGLGFNLTELSESDVHRGFQHLLHTLNLPGHGLETRVGSALFLSHNLKFLAKFLNDTMAVYEAKLFHTNFYDTVGTIQLINDHVKKETRGKIVDLVSELKKDVLMVLVNYIYFKALWEKPFISSRTTPKDFYVDENTTVRVPMMLQDQEHHWYLHDRYLPCSVLRMDYKGDATVFFILPNQGKMREIEEVLTPEMLMRWNNLLRKRNFYKKLELHLPKFSISGSYVLDQILPRLGFTDLFSKWADLSGITKQQKLEASKSFHKATLDVDEAGTEAAAATSFAIKFFSAQTNRHILRFNRPFLVVIFSTSTQSVLFLGKVVDPTKP","proteinLength":"Full Length","predictedMolecularWeight":"46.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":427,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P29622","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallistatin also known as PI-4 is a serine proteinase inhibitor with a mass of approximately 52 kDa. You find it mainly in the liver as part of the serpin superfamily. This protein acts mechanically by inhibiting the tissue kallikrein-kinin system which involves proteolytic processes that regulate vascular homeostasis and blood pressure. In addition to the liver kallistatin is expressed in endothelial cells and various human tissues.
Biological function summary

Kallistatin participates in inhibiting angiogenesis and inflammation impacting cellular behavior and responses. As a serpin it functions uniquely in forming stable complexes with target enzymes like tissue kallikrein. Through this mechanism kallistatin contributes to maintaining vascular integrity and preventing excessive vascular growth or inflammation that could lead to pathology.

Pathways

The inhibitory effects of kallistatin integrate into the kallikrein-kinin system and the renin-angiotensin system. In the kallikrein-kinin system kallistatin regulates processes involving vascular tone and fluid balance. It interacts with proteins such as bradykinin mediating inflammatory responses and endothelial cell function through these key pathways.

Researchers link kallistatin with conditions like hypertension and cancer. Its role in inhibiting angiogenesis and inflammation helps understand its protective effects against tumor growth and metastasis. In hypertension kallistatin's relationship with the renin-angiotensin system connects it to other proteins like angiotensin II influencing blood pressure regulation and vascular health.
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Specifications

Form

Lyophilized

Additional notes

ab153781 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE. It is lyophilized from an 0.2 µM filtered solution.

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General info

Function

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

The N-terminus is blocked.

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Product protocols

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Target data

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
See full target information SERPINA4
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