Recombinant Human KASH5 protein is a Human Full Length protein, in the 1 to 562 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M D L P E G P V G G P T A E M Y L R E R P E E A R L G M P V S L E E Q I L N S T F E A C D P Q R T G T V A V A Q V L A Y L E A V T G Q G P Q D A R L Q T L A N S L D P N G E G P K A T V D L D T F L V V M R D W I A A C Q L H G G L E L E E E T A F Q G A L T S Q Q L P S G C P E A E E P A N L E S F G G E D P R P E L Q A T A D L L S S L E D L E L S N R R L V G E N A K L Q R S M E T A E E G S A R L G E E I L A L R K Q L H S T Q Q A L Q F A K A M D E E L E D L K T L A R S L E E Q N R S L L A Q A R Q A E K E Q Q H L V A E M E T L Q E E N G K L L A E R D G V K K R S Q E L A M E K D T L K R Q L F E C E H L I C Q R D T I L S E R T R D V E S L A Q T L E E Y R V T T Q E L R L E I S R L E E Q L S Q T Y E G P D E L P E G A Q L R R V G W T E L L P P S L G L E I E A I R Q K Q E V A T A D L S N P L C G V W Q W E E V I H E T S E E T E F P S E A P A G G Q R N F Q G E P A H P E E G R K E P S M W L T R R E E E E D A E S Q V T A D L P V P L G A P R P G D I P E N P P E R P A R R E L Q Q A L V P V M K K L V P V R R R A W G Q L C L P P Q R L R V T R H P L I P A P V L G L L L L L L L S V L L L G P S P P P T W P H L Q L C Y L Q P P P V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for telomere attachment to nuclear envelope in the prophase of meiosis (PubMed:35587281). Required for rapid telomere prophase movements implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for homolog pairing during meiotic prophase in spermatocytes and probably oocytes. Essential for male and female gametogenesis (PubMed:35587281). Recruits cytoplasmic dynein to telomere attachment sites at the nuclear envelope in spermatocytes. In oocytes is involved in meiotic resumption and spindle formation.
Alternative names
CCDC155, KASH5, Protein KASH5, Coiled-coil domain-containing protein 155, KASH domain-containing protein 5
Recommended products
Recombinant Human KASH5 protein is a Human Full Length protein, in the 1 to 562 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q8N6L0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D L P E G P V G G P T A E M Y L R E R P E E A R L G M P V S L E E Q I L N S T F E A C D P Q R T G T V A V A Q V L A Y L E A V T G Q G P Q D A R L Q T L A N S L D P N G E G P K A T V D L D T F L V V M R D W I A A C Q L H G G L E L E E E T A F Q G A L T S Q Q L P S G C P E A E E P A N L E S F G G E D P R P E L Q A T A D L L S S L E D L E L S N R R L V G E N A K L Q R S M E T A E E G S A R L G E E I L A L R K Q L H S T Q Q A L Q F A K A M D E E L E D L K T L A R S L E E Q N R S L L A Q A R Q A E K E Q Q H L V A E M E T L Q E E N G K L L A E R D G V K K R S Q E L A M E K D T L K R Q L F E C E H L I C Q R D T I L S E R T R D V E S L A Q T L E E Y R V T T Q E L R L E I S R L E E Q L S Q T Y E G P D E L P E G A Q L R R V G W T E L L P P S L G L E I E A I R Q K Q E V A T A D L S N P L C G V W Q W E E V I H E T S E E T E F P S E A P A G G Q R N F Q G E P A H P E E G R K E P S M W L T R R E E E E D A E S Q V T A D L P V P L G A P R P G D I P E N P P E R P A R R E L Q Q A L V P V M K K L V P V R R R A W G Q L C L P P Q R L R V T R H P L I P A P V L G L L L L L L L S V L L L G P S P P P T W P H L Q L C Y L Q P P P V
- Accession
- Q8N6L0
- Protein length
- Full Length
- Amino acids
- 1 to 562
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for telomere attachment to nuclear envelope in the prophase of meiosis (PubMed:35587281). Required for rapid telomere prophase movements implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for homolog pairing during meiotic prophase in spermatocytes and probably oocytes. Essential for male and female gametogenesis (PubMed:35587281). Recruits cytoplasmic dynein to telomere attachment sites at the nuclear envelope in spermatocytes. In oocytes is involved in meiotic resumption and spindle formation.
- Subcellular localisation
- Nucleus outer membrane, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CCDC155.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KASH5 also known as the Kinesin-Associated Scaffold Protein 5 is a protein with a molecular mass of approximately 87 kDa. It operates mechanistically by playing a role in the nuclear envelope as part of the LINC complex essential for nuclear-cytoskeletal coupling. This protein is mainly expressed in the testes of mammals indicating a role in reproductive biology. As a component of the outer nuclear membrane KASH5 connects to microtubules and regulates their dynamics.
- Biological function summary
KASH5 plays a critical role in meiotic chromosome movements ensuring proper alignment and segregation during cell division. It acts as part of the larger machinery that includes the LINC complex which bridges the nuclear lamina with the cytoskeleton. KASH5 works closely with SUN proteins facilitating dynein and kinesin motor activity. This association aids in the transmission of mechanical cues necessary for cellular processes.
- Pathways
KASH5 integrates into the broader landscape of the meiotic cell cycle regulation and nuclear envelope positioning. It interacts prominently within the nuclear dynamics pathway relying on its cooperation with SUN2 and Nesprin proteins. Through these pathways it modulates chromosome positioning and facilitates kinetochore function during cell division ensuring genetic stability.
- Associated diseases and disorders
KASH5 mutations or dysfunctions have been implicated in fertility issues particularly impacting spermatogenesis. Moreover alterations in its function connect to certain muscular dystrophies as it affects muscle cell nuclei positioning. Its relationship with disorders often involves interactions with proteins like Lamin A/C which impacts nuclear integrity in neuromuscular diseases.
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1 product image
SDS-PAGE - Recombinant Human KASH5 protein (ab165568)
ab165568 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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