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AB93687

Recombinant Human KAT1 / HAT1 protein

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Recombinant Human KAT1 / HAT1 protein is a Human Fragment protein, in the 20 to 341 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

KAT1, HAT1, Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1

1 Images
SDS-PAGE - Recombinant Human KAT1 / HAT1 protein (AB93687)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KAT1 / HAT1 protein (AB93687)

15% SDS-PAGE showing ab93687 at approximately 40 kDa (3 μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O14929

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":341,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O14929","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein KAT1 also known as HAT1 (Histone Acetyltransferase 1) functions as an enzyme responsible for acetylating newly synthesized histone proteins. It has a mass of approximately 46 kDa. KAT1 is mostly expressed in the nucleus of cells where it modifies histones by adding acetyl groups to the N-terminal lysines a process essential for chromatin modification. The enzyme is important for facilitating transcriptional activation by altering chromatin structure and is widely observed in various eukaryotic organisms.
Biological function summary

KAT1 acts as a part of the histone acetyltransferase complex which plays a major role in regulating gene expression. By acetylating histone proteins it reduces chromatin compaction and allows gene transcription machinery access to DNA. This alteration can significantly influence cellular processes such as cell cycle regulation and DNA repair. As KAT1 interacts with several protein partners including other acetylation enzymes it serves as an important player in managing chromatin dynamics and maintaining genomic integrity.

Pathways

The role of KAT1 extends to involvement in the cell cycle and DNA damage repair pathways. In the cell cycle pathway KAT1 facilitates transcription required for progression through various phases of the cycle. In DNA damage response pathways KAT1 contributes to repairing DNA lesions by modifying chromatin to allow repair machinery access. It interacts with proteins such as p300 and CBP which are also acetyltransferases to modulate these pathways effectively and ensure cellular responses to DNA insults are adequate.

KAT1's function connects with cancer and neurodegenerative diseases due to its role in gene regulation and DNA repair. In cancer aberrant acetylation by KAT1 can lead to unregulated cell division and tumor formation while in neurodegenerative disorders impaired KAT1 activity might contribute to neuronal damage and death due to faulty DNA repair. The protein interacts with p53 another key player in both pathways impacting disease progression by modulating tumor suppressor functions and ensuring cellular stability under stress.
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Specifications

Form

Liquid

Additional notes

ab93687 is purified using conventional chromatography techniques.

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General info

Function

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed : 20953179, PubMed : 23653357, PubMed : 31278053, PubMed : 32081014). Coordinates histone production and acetylation via H4 promoter binding (PubMed : 31278053). Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed : 11585814, PubMed : 22615379). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed : 31278053). Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed : 23653357). In addition, acetylates other substrates such as chromatin-related proteins (PubMed : 32081014). Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed : 31812350).. (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.

Sequence similarities

Belongs to the HAT1 family.

Post-translational modifications

Phosphorylated by AMPK at Ser-190; phosphorylation increases HAT1 activity.

Subcellular localisation

Nucleus matrix

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Product protocols

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Target data

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed : 20953179, PubMed : 23653357, PubMed : 31278053, PubMed : 32081014). Coordinates histone production and acetylation via H4 promoter binding (PubMed : 31278053). Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed : 11585814, PubMed : 22615379). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed : 31278053). Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed : 23653357). In addition, acetylates other substrates such as chromatin-related proteins (PubMed : 32081014). Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed : 31812350).. (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.
See full target information HAT1
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Assay Kits

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