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AB56275

Recombinant Human KAT2B / PCAF protein

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Recombinant Human KAT2B / PCAF protein is a Human Fragment protein, in the 431 to 832 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

View Alternative Names

PCAF, KAT2B, Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, Spermidine acetyltransferase KAT2B, Histone acetylase PCAF, P/CAF

1 Images
SDS-PAGE - Recombinant Human KAT2B / PCAF protein (AB56275)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KAT2B / PCAF protein (AB56275)

ab56275 on SDS-PAGE. The extra band found on the SDS gel may be a truncated form of PCAF, but because we have not yet done a Western Blot on this product, the identity is still unconfirmed.

Key facts

Purity

>70% Densitometry

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q92831

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":832,"aminoAcidStart":431,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q92831","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT2B also known as PCAF (p300/CBP-associated factor) is a histone acetyltransferase protein with a mass of approximately 94 kDa. It plays a role in chromatin remodeling by acetylating lysines on histone tails which facilitates transcriptional activation. KAT2B is expressed ubiquitously but higher expressions occur in tissues with active transcription such as the brain and testes. KAT2B/PCAF can also interact with transcription regulators adding another layer to its role in gene expression regulation.
Biological function summary

KAT2B/PCAF participates in modifying chromatin structure and impacting transcription. It forms part of various complexes most notably a complex with other proteins like p300 and CBP which have acetyltransferase activity too. Through modifying histones KAT2B/PCAF influences gene expression patterns critical for cell cycle regulation and differentiation. It also acetylates non-histone proteins playing roles in diverse biological processes beyond transcription.

Pathways

KAT2B/PCAF integrates into the regulation of cell cycle and apoptosis pathways. One of the major pathways it involves in is the p53 signaling pathway which is important for cell cycle arrest and apoptosis in response to DNA damage. KAT2B/PCAF works with proteins like p53 and HDACs (histone deacetylases) to fine-tune the balance between acetylation and deacetylation of histones and other proteins influencing cell cycle progression and cellular response to stress signals.

KAT2B/PCAF has associations with cancer and neurodegenerative diseases. Its capability to regulate cell cycle and apoptosis connects it to cancer biology where altered expression or activity might contribute to unchecked cell proliferation. Variations in KAT2B/PCAF activity also link to neurodegenerative disorders like Huntington’s disease. The protein interacts with other factors like p53 and SMAD transcription factors in contexts relevant to these diseases suggesting its roles in pathways critical for disease development or progression.
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Specifications

Form

Liquid

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General info

Function

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed : 8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed : 8945521). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed : 21131905). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed : 10675335, PubMed : 23001180, PubMed : 23932781, PubMed : 26867678, PubMed : 27796307, PubMed : 29174768, PubMed : 9707565). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed : 8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators : NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed : 14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed : 29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed : 27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed : 26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed : 23001180). Also acetylates spermidine (PubMed : 27389534).. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Sequence similarities

Belongs to the acetyltransferase family. GCN5 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed : 8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed : 8945521). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed : 21131905). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed : 10675335, PubMed : 23001180, PubMed : 23932781, PubMed : 26867678, PubMed : 27796307, PubMed : 29174768, PubMed : 9707565). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed : 8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators : NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed : 14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed : 29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed : 27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed : 26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed : 23001180). Also acetylates spermidine (PubMed : 27389534).. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
See full target information KAT2B
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