Recombinant Human KAT2B / PCAF protein is a Human Fragment protein, in the 431 to 832 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >70% Densitometry
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed:8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed:8945521). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed:21131905). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed:10675335, PubMed:23001180, PubMed:23932781, PubMed:26867678, PubMed:27796307, PubMed:29174768, PubMed:9707565). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed:8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed:29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed:27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed:26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed:23001180). Also acetylates spermidine (PubMed:27389534). (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Alternative names
PCAF, KAT2B, Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, Spermidine acetyltransferase KAT2B, Histone acetylase PCAF, P/CAF
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Recombinant Human KAT2B / PCAF protein is a Human Fragment protein, in the 431 to 832 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q92831-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- Q92831
- Protein length
- Fragment
- Predicted molecular weight
- 69 kDa
- Amino acids
- 431 to 832
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed:8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed:8945521). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed:21131905). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed:10675335, PubMed:23001180, PubMed:23932781, PubMed:26867678, PubMed:27796307, PubMed:29174768, PubMed:9707565). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed:8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed:29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed:27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed:26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed:23001180). Also acetylates spermidine (PubMed:27389534).
- Sequence similarities
Belongs to the acetyltransferase family. GCN5 subfamily.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KAT2B also known as PCAF (p300/CBP-associated factor) is a histone acetyltransferase protein with a mass of approximately 94 kDa. It plays a role in chromatin remodeling by acetylating lysines on histone tails which facilitates transcriptional activation. KAT2B is expressed ubiquitously but higher expressions occur in tissues with active transcription such as the brain and testes. KAT2B/PCAF can also interact with transcription regulators adding another layer to its role in gene expression regulation.
- Biological function summary
KAT2B/PCAF participates in modifying chromatin structure and impacting transcription. It forms part of various complexes most notably a complex with other proteins like p300 and CBP which have acetyltransferase activity too. Through modifying histones KAT2B/PCAF influences gene expression patterns critical for cell cycle regulation and differentiation. It also acetylates non-histone proteins playing roles in diverse biological processes beyond transcription.
- Pathways
KAT2B/PCAF integrates into the regulation of cell cycle and apoptosis pathways. One of the major pathways it involves in is the p53 signaling pathway which is important for cell cycle arrest and apoptosis in response to DNA damage. KAT2B/PCAF works with proteins like p53 and HDACs (histone deacetylases) to fine-tune the balance between acetylation and deacetylation of histones and other proteins influencing cell cycle progression and cellular response to stress signals.
- Associated diseases and disorders
KAT2B/PCAF has associations with cancer and neurodegenerative diseases. Its capability to regulate cell cycle and apoptosis connects it to cancer biology where altered expression or activity might contribute to unchecked cell proliferation. Variations in KAT2B/PCAF activity also link to neurodegenerative disorders like Huntington’s disease. The protein interacts with other factors like p53 and SMAD transcription factors in contexts relevant to these diseases suggesting its roles in pathways critical for disease development or progression.
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1 product image
SDS-PAGE - Recombinant Human KAT2B / PCAF protein (ab56275)
ab56275 on SDS-PAGE.
The extra band found on the SDS gel may be a truncated form of PCAF, but because we have not yet done a Western Blot on this product, the identity is still unconfirmed.
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