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AB135219

Recombinant Human KAT4 / TBP Associated Factor 1 protein

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Recombinant Human KAT4 / TBP Associated Factor 1 protein is a Human Fragment protein, in the 1032 to 1872 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB.

View Alternative Names

BA2R, CCG1, CCGS, TAF2A, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250

1 Images
SDS-PAGE - Recombinant Human KAT4 / TBP Associated Factor 1 protein (AB135219)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KAT4 / TBP Associated Factor 1 protein (AB135219)

SDS-PAGE analysis of ab135219.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P21675

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE","proteinLength":"Fragment","predictedMolecularWeight":"155 kDa","actualMolecularWeight":null,"aminoAcidEnd":1872,"aminoAcidStart":1032,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P21675","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT4 also known as TBP Associated Factor 1 (TAF1) is a protein with a mass of around 240 kDa. It functions mechanically as part of the transcription machinery where it acts as an important component of the transcription factor IID (TFIID) complex. This protein is expressed in various tissues with a significant expression in the liver. TAF1 is involved in the regulation of gene expression acting as a bridge between specific DNA sequences and the general transcription factors that promote RNA polymerase II activity.
Biological function summary

TAF1 is an integral part of the TFIID complex which consists of multiple subunits. It plays a critical role in the initiation of gene transcription by facilitating the binding of the TFIID complex to RNA polymerase II and other associated factors. TAF1 contributes to the accurate transcription start site selection and the regulation of promoter activity influencing gene expression across diverse cell types.

Pathways

The role of TAF1 fits into the transcription regulation and RNA polymerase II-mediated pathways. It collaborates with proteins like TBP (TATA-binding protein) to initiate transcription. TAF1's kinase activity also involves phosphorylation processes that are part of transcription regulation mechanisms connecting it to broader signaling pathways within the cell.

TAF1 has associations with neurodevelopmental and degenerative disorders such as Parkinson's disease. Mutations or dysregulation in TAF1 can lead to abnormal transcriptional activity which impacts neuronal development and survival. The protein's interaction with other transcription-related proteins including TBP provides further insight into how transcriptional misregulation can contribute to these diseases.
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Specifications

Form

Liquid

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General info

Function

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed : 25412659, PubMed : 27007846, PubMed : 33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed : 33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed : 25412659, PubMed : 8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed : 25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed : 25412659). Possesses DNA-binding activity (PubMed : 25412659). Essential for progression of the G1 phase of the cell cycle (PubMed : 11278496, PubMed : 15053879, PubMed : 2038334, PubMed : 8450888, PubMed : 8625415, PubMed : 9660973, PubMed : 9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed : 15870300).

Sequence similarities

Belongs to the TAF1 family.

Post-translational modifications

Phosphorylated by casein kinase II in vitro.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed : 25412659, PubMed : 27007846, PubMed : 33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed : 33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed : 25412659, PubMed : 8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed : 25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed : 25412659). Possesses DNA-binding activity (PubMed : 25412659). Essential for progression of the G1 phase of the cell cycle (PubMed : 11278496, PubMed : 15053879, PubMed : 2038334, PubMed : 8450888, PubMed : 8625415, PubMed : 9660973, PubMed : 9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed : 15870300).
See full target information TAF1
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