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AB105858

Recombinant Human KAT9 / Elp3 protein

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Recombinant Human KAT9 / Elp3 protein is a Human Full Length protein, in the 1 to 547 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for WB.

View Alternative Names

Elongator complex protein 3, hELP3, tRNA uridine(34) acetyltransferase, ELP3

1 Images
SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (AB105858)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (AB105858)

ab105858 was determined to be >75% pure by densitometry.

Key facts

Purity

>75% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

WB

applications

Biologically active

No

Accession

Q9H9T3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.307% Glutathione, 0.29% Sodium chloride, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":547,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9H9T3","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT9 also known as Elp3 is an important catalytic component of the elongator complex which is involved in transcriptional elongation. This protein possesses histone acetyltransferase (HAT) activity enabling it to modify histones by acetylating lysine residues a process important for chromatin remodeling and regulation of gene expression. KAT9 is composed of around 55 kDa and is ubiquitously expressed in various tissues reflecting its essential role in diverse cellular processes.
Biological function summary

KAT9/Elp3 is integral in regulating normal cellular growth and differentiation. It functions as part of the elongator complex which consists of six subunits playing a significant role in tRNA modification and mRNA translation elongation. By supporting the translational machinery Elp3 influences cell cycle progression which is critical for proper cellular functions and organism development.

Pathways

The functions of KAT9 hold relevance in the transcriptional and translational regulation pathways. It contributes significantly to the RNA polymerase II-mediated transcription elongation pathway. In addition there is interaction with proteins such as ELP1 and ELP2 with which it forms complexes facilitating various stages of RNA processing and modification. The activity of these complexes impacts cellular responses under stress conditions regulating gene expression for adaptation.

Alterations in KAT9 expression or function are associated with neurodegenerative diseases and cancers. Defects in the elongator complex involving Elp3 relate to familial dysautonomia a disorder affecting the autonomic nervous system. Additionally KAT9 is connected to colorectal cancer where its dysregulation alters transcriptional dynamics potentially contributing to tumor progression. The interplay between Elp3 and other elongator components like Elp1 highlights its significance in maintaining normal cellular homeostasis and preventing pathological states.
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Specifications

Form

Liquid

Additional notes

ab105858 was determined to be >75% pure by densitometry. Affinity purified.

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General info

Function

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed : 29415125). Involved in neurogenesis : regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed : 19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).

Sequence similarities

Belongs to the ELP3 family.

Post-translational modifications

Tyrosine-phosphorylated; phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability (PubMed:31341009). Also serine/threonine-phosphorylated (PubMed:31341009).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed : 29415125). Involved in neurogenesis : regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed : 19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).
See full target information ELP3
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