Recombinant human KCNE2 protein (His tag) is a Human Full Length protein, in the 1 to 123 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S T L S N F T Q T L E D V F R R I F I T Y M D N W R Q N T T A E Q E A L Q A K V D A E N F Y Y V I L Y L M V M I G M F S F I I V A I L V S T V K S K R R E H S N D P Y H Q Y I V E D W Q E K Y K S Q I L N L E E S K A T I H E N I G A A G F K M S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ancillary protein that functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta subunits (PubMed:10219239, PubMed:11034315, PubMed:11101505, PubMed:12185453, PubMed:20533308). KCNE2 beta subunit modulates the gating kinetics and enhances stability of the channel complex (PubMed:10219239, PubMed:11034315, PubMed:11101505, PubMed:12185453, PubMed:20533308). Alters the gating of the delayed rectifier Kv channel containing KCNB1 alpha subunit (PubMed:11101505, PubMed:20533308). Associates with KCNH2/HERG alpha subunit Kv channel to form the rapidly activating component of the delayed rectifying potassium current (IKr) in heart (PubMed:10219239, PubMed:12185453). May associate with KCNQ2 and/or KCNQ3 alpha subunits to modulate the native M-type current (PubMed:11034315). May associate with HCN1 and HCN2 channel subunits to increase potassium current (By similarity). Forms a heterooligomer complex with KCNQ1/KVLQT1 alpha subunits which leads to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability (By similarity).
Alternative names
Potassium voltage-gated channel subfamily E member 2, MinK-related peptide 1, Minimum potassium ion channel-related peptide 1, Potassium channel subunit beta MiRP1, MiRP1, KCNE2
Recombinant human KCNE2 protein (His tag) is a Human Full Length protein, in the 1 to 123 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9Y6J6-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate
Sequence info
Amino acid sequence
- M S T L S N F T Q T L E D V F R R I F I T Y M D N W R Q N T T A E Q E A L Q A K V D A E N F Y Y V I L Y L M V M I G M F S F I I V A I L V S T V K S K R R E H S N D P Y H Q Y I V E D W Q E K Y K S Q I L N L E E S K A T I H E N I G A A G F K M S P
- Accession
- Q9Y6J6
- Protein length
- Full Length
- Predicted molecular weight
- 17.3 kDa
- Amino acids
- 1 to 123
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Ancillary protein that functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta subunits (PubMed:10219239, PubMed:11034315, PubMed:11101505, PubMed:12185453, PubMed:20533308). KCNE2 beta subunit modulates the gating kinetics and enhances stability of the channel complex (PubMed:10219239, PubMed:11034315, PubMed:11101505, PubMed:12185453, PubMed:20533308). Alters the gating of the delayed rectifier Kv channel containing KCNB1 alpha subunit (PubMed:11101505, PubMed:20533308). Associates with KCNH2/HERG alpha subunit Kv channel to form the rapidly activating component of the delayed rectifying potassium current (IKr) in heart (PubMed:10219239, PubMed:12185453). May associate with KCNQ2 and/or KCNQ3 alpha subunits to modulate the native M-type current (PubMed:11034315). May associate with HCN1 and HCN2 channel subunits to increase potassium current (By similarity). Forms a heterooligomer complex with KCNQ1/KVLQT1 alpha subunits which leads to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability (By similarity).
- Sequence similarities
Belongs to the potassium channel KCNE family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C, -80°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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1 product image
SDS-PAGE - Recombinant human KCNE2 protein (His tag) (ab314528)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Downloads
Product protocols
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