Recombinant human KCNJ16 protein (His tag) is a Human Full Length protein, in the 1 to 418 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S Y Y G S S Y H I I N A D A K Y P G Y P P E H I I A E K R R A R R R L L H K D G S C N V Y F K H I F G E W G S Y V V D I F T T L V D T K W R H M F V I F S L S Y I L S W L I F G S V F W L I A F H H G D L L N D P D I T P C V D N V H S F T G A F L F S L E T Q T T I G Y G Y R C V T E E C S V A V L M V I L Q S I L S C I I N T F I I G A A L A K M A T A R K R A Q T I R F S Y F A L I G M R D G K L C L M W R I G D F R P N H V V E G T V R A Q L L R Y T E D S E G R M T M A F K D L K L V N D Q I I L V T P V T I V H E I D H E S P L Y A L D R K A V A K D N F E I L V T F I Y T G D S T G T S H Q S R S S Y V P R E I L W G H R F N D V L E V K R K Y Y K V N C L Q F E G S V E V Y A P F C S A K Q L D W K D Q Q L H I E K A P P V R E S C T S D T K A R R R S F S A V A I V S S C E N P E E T T T S A T H E Y R E T P Y Q K A L L T L N R I S V E S Q M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (PubMed:24561201).
Alternative names
Inward rectifier potassium channel 16, Inward rectifier K(+) channel Kir5.1, KCNJ16
Recombinant human KCNJ16 protein (His tag) is a Human Full Length protein, in the 1 to 418 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NPI9-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate
Sequence info
Amino acid sequence
- M S Y Y G S S Y H I I N A D A K Y P G Y P P E H I I A E K R R A R R R L L H K D G S C N V Y F K H I F G E W G S Y V V D I F T T L V D T K W R H M F V I F S L S Y I L S W L I F G S V F W L I A F H H G D L L N D P D I T P C V D N V H S F T G A F L F S L E T Q T T I G Y G Y R C V T E E C S V A V L M V I L Q S I L S C I I N T F I I G A A L A K M A T A R K R A Q T I R F S Y F A L I G M R D G K L C L M W R I G D F R P N H V V E G T V R A Q L L R Y T E D S E G R M T M A F K D L K L V N D Q I I L V T P V T I V H E I D H E S P L Y A L D R K A V A K D N F E I L V T F I Y T G D S T G T S H Q S R S S Y V P R E I L W G H R F N D V L E V K R K Y Y K V N C L Q F E G S V E V Y A P F C S A K Q L D W K D Q Q L H I E K A P P V R E S C T S D T K A R R R S F S A V A I V S S C E N P E E T T T S A T H E Y R E T P Y Q K A L L T L N R I S V E S Q M
- Accession
- Q9NPI9
- Protein length
- Full Length
- Predicted molecular weight
- 50.8 kDa
- Amino acids
- 1 to 418
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (PubMed:24561201).
- Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ16 subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C, -80°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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1 product image
SDS-PAGE - Recombinant human KCNJ16 protein (His tag) (ab314527)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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