Recombinant Human KCNN4 protein

Specifications

Form

Liquid

General info

Function

Intermediate conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening (PubMed : 10026195, PubMed : 10961988, PubMed : 11425865, PubMed : 15831468, PubMed : 17157250, PubMed : 18796614, PubMed : 26148990, PubMed : 9326665, PubMed : 9380751, PubMed : 9407042). The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 25 picosiemens (PubMed : 9326665, PubMed : 9380751, PubMed : 9407042). Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV (PubMed : 9326665, PubMed : 9380751, PubMed : 9407042). Controls calcium influx during vascular contractility by being responsible of membrane hyperpolarization induced by vasoactive factors in proliferative vascular smooth muscle cell types (By similarity). Following calcium influx, the consecutive activation of KCNN4 channel leads to a hyperpolarization of the cell membrane potential and hence an increase of the electrical driving force for further calcium influx promoting sustained calcium entry in response to stimulation with chemotactic peptides (PubMed : 26418693). Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed : 17157250, PubMed : 18796614). Plays a role in the late stages of EGF-induced macropinocytosis through activation by PI(3)P (PubMed : 24591580).

Sequence similarities

Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

Post-translational modifications

Phosphorylation at His-358 by NDKB activates the intermediate conductance calcium-activated potassium channel activity, and conversely it's dephosphorylation by PHPT1 inhibits this activity.