Recombinant human KDM2A protein (Active) is a Human Fragment protein, in the 2 to 700 aa range, expressed in Baculovirus infected Sf9, with >=80% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Avi tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
E P E E E R I R Y S Q R L R G T M R R R Y E D D G I S D D E I E G K R T F D L E E K L H T N K Y N A N F V T F M E G K D F N V E Y I Q R G G L R D P L I F K N S D G L G I K M P D P D F T V N D V K M C V G S R R M V D V M D V N T Q K G I E M T M A Q W T R Y Y E T P E E E R E K L Y N V I S L E F S H T R L E N M V Q R P S T V D F I D W V D N M W P R H L K E S Q T E S T N A I L E M Q Y P K V Q K Y C L M S V R G C Y T D F H V D F G G T S V W Y H I H Q G G K V F W L I P P T A H N L E L Y E N W L L S G K Q G D I F L G D R V S D C Q R I E L K Q G Y T F V I P S G W I H A V Y T P T D T L V F G G N F L H S F N I P M Q L K I Y N I E D R T R V P N K F R Y P F Y Y E M C W Y V L E R Y V Y C I T N R S H L T K E F Q K E S L S M D L E L N G L E S G N G D E E A V D R E P R R L S S R R S V L T S P V A N G V N L D Y D G L G K T C R S L P S L K K T L A G D S S S D C S R G S H N G Q V W D P Q C A P R K D R Q V H L T H F E L E G L R C L V D K L E S L P L H K K C V P T G I E D E D A L I A D V K I L L E E L A N S D P K L A L T G V P I V Q W P K R D K L K F P T R P K V R V P T I P I T K P H T M K P A P R L T P V R P A A A S P I V S G A R R R R V R C R K C K A C V Q G E C G V C H Y C R D M K K F G G P G R M K Q S C V L R Q C L A P R L P H S V T C S L C G E V D Q N E E T Q D F E K K L M E C C I C N E I V H P G C L Q M D G E G L L N E E L P N C W E C P K C Y Q E D S S E K A Q K R K M E E S D E E A V Q A K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310).
Alternative names
CXXC8, FBL11, FBL7, FBXL11, JHDM1A, KIAA1004, KDM2A, Lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A
Recommended products
Recombinant human KDM2A protein (Active) is a Human Fragment protein, in the 2 to 700 aa range, expressed in Baculovirus infected Sf9, with >=80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Avi tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 0.01 pmole/min/μg.
- Accession
- Q9Y2K7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- E P E E E R I R Y S Q R L R G T M R R R Y E D D G I S D D E I E G K R T F D L E E K L H T N K Y N A N F V T F M E G K D F N V E Y I Q R G G L R D P L I F K N S D G L G I K M P D P D F T V N D V K M C V G S R R M V D V M D V N T Q K G I E M T M A Q W T R Y Y E T P E E E R E K L Y N V I S L E F S H T R L E N M V Q R P S T V D F I D W V D N M W P R H L K E S Q T E S T N A I L E M Q Y P K V Q K Y C L M S V R G C Y T D F H V D F G G T S V W Y H I H Q G G K V F W L I P P T A H N L E L Y E N W L L S G K Q G D I F L G D R V S D C Q R I E L K Q G Y T F V I P S G W I H A V Y T P T D T L V F G G N F L H S F N I P M Q L K I Y N I E D R T R V P N K F R Y P F Y Y E M C W Y V L E R Y V Y C I T N R S H L T K E F Q K E S L S M D L E L N G L E S G N G D E E A V D R E P R R L S S R R S V L T S P V A N G V N L D Y D G L G K T C R S L P S L K K T L A G D S S S D C S R G S H N G Q V W D P Q C A P R K D R Q V H L T H F E L E G L R C L V D K L E S L P L H K K C V P T G I E D E D A L I A D V K I L L E E L A N S D P K L A L T G V P I V Q W P K R D K L K F P T R P K V R V P T I P I T K P H T M K P A P R L T P V R P A A A S P I V S G A R R R R V R C R K C K A C V Q G E C G V C H Y C R D M K K F G G P G R M K Q S C V L R Q C L A P R L P H S V T C S L C G E V D Q N E E T Q D F E K K L M E C C I C N E I V H P G C L Q M D G E G L L N E E L P N C W E C P K C Y Q E D S S E K A Q K R K M E E S D E E A V Q A K
- Accession
- Q9Y2K7
- Protein length
- Fragment
- Predicted molecular weight
- 82 kDa
- Amino acids
- 2 to 700
- Nature
- Recombinant
- Tags
- Avi tag N-Terminus, DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310).
- Sequence similarities
Belongs to the JHDM1 histone demethylase family.
- Post-translational modifications
Mono-ADP-ribosylated at Arg-1020 in response to DNA damage, leading to displacement from chromatin, resulting in increased dimethylation of histone H3 at 'Lys-36'.
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Terms & Conditions.
2 product images
Functional Studies - Recombinant human KDM2A protein (Active) (ab271507)
Specific activity of ab271507.
SDS-PAGE - Recombinant human KDM2A protein (Active) (ab271507)
SDS-PAGE analysis of 3.9 μg ab271507.
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Product protocols
- Visit the general protocols
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