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AB196435

Recombinant human KDM2B protein

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Recombinant human KDM2B protein is a Human Fragment protein, in the 1 to 650 aa range, expressed in Baculovirus infected Sf9 cells, with >83%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CXXC2, FBL10, FBXL10, JHDM1B, NDY1, PCCX2, KDM2B, Lysine-specific demethylase 2B, CXXC-type zinc finger protein 2, F-box and leucine-rich repeat protein 10, F-box protein FBL10, F-box/LRR-repeat protein 10, JmjC domain-containing histone demethylation protein 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, Protein-containing CXXC domain 2, [Histone-H3]-lysine-36 demethylase 1B, Protein JEMMA

3 Images
Functional Studies - Recombinant human KDM2B protein (AB196435)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM2B protein (AB196435)

Specific activity of ab196435

SDS-PAGE - Recombinant human KDM2B protein (AB196435)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM2B protein (AB196435)

SDS-PAGE analysis of 2.4 μg of ab196435 on a 4-20% SDS gel stained with Coomassie.

SDS-PAGE - Recombinant human KDM2B protein (AB196435)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM2B protein (AB196435)

SDS-PAGE analysis of 2.8 μg of ab196435 on a 4-20% SDS gel stained with Coomassie.

Key facts

Purity

>83% HPLC

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Enzyme was incubated with 0.4 μM substrate in HEPES-containing JMJ assay buffer SA and SA- (without KG) in a 10-μl reaction for 180 min at 30˚C. 5 μl of Anti-Rabbit acceptor beads (1:250) and 5 μl of rabbit polyclonal antibody (1:200) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 μl) and mixture was further incubated for 10 min at RT before counts were measured.

Accession

Q8NHM5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNAIAEMKYPKVKKYCLMSVKGCFTDFHIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSIDGFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTPSEDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDPSLAITGVPVVTWPKKTPKNRAVGRPKGKLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQ","proteinLength":"Fragment","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":650,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8NHM5","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

>=85% by SDS-PAGE. Affinity purified.

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General info

Function

Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36' (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation (PubMed : 16362057, PubMed : 17994099). May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (Probable).

Sequence similarities

Belongs to the JHDM1 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36' (PubMed : 16362057, PubMed : 17994099, PubMed : 26237645). Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation (PubMed : 16362057, PubMed : 17994099). May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (Probable).
See full target information KDM2B
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