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AB198149

Recombinant human KDM4B / JMJD2B protein

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Recombinant human KDM4B / JMJD2B protein is a Human Fragment protein, in the 1 to 500 aa range, expressed in Baculovirus infected Sf9 cells, with >41%, suitable for SDS-PAGE, FuncS.

View Alternative Names

JHDM3B, JMJD2B, KIAA0876, KDM4B, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, [histone H3]-trimethyl-L-lysine(9) demethylase 4B

2 Images
Functional Studies - Recombinant human KDM4B / JMJD2B protein (AB198149)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM4B / JMJD2B protein (AB198149)

Specific Activity of ab198149.

SDS-PAGE - Recombinant human KDM4B / JMJD2B protein (AB198149)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM4B / JMJD2B protein (AB198149)

4-20% SDS-PAGE analysis of ab198149 (1.97μg) with Coomassie staining.

Key facts

Purity

>41% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.09 pmole/min/μg.

Assay Conditions:

10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and KDM4B / JMJD2B (20 – 100 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Accession

O94953

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTALTSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALLEEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKAKSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEES","proteinLength":"Fragment","predictedMolecularWeight":"57 kDa","actualMolecularWeight":null,"aminoAcidEnd":500,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O94953","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed : 16603238, PubMed : 28262558). Plays a critical role in the development of the central nervous system (CNS).

Sequence similarities

Belongs to the JHDM3 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed : 16603238, PubMed : 28262558). Plays a critical role in the development of the central nervous system (CNS).
See full target information KDM4B
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