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AB198159

Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus)

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(1 Publication)

Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus) is a Human Fragment protein, in the 2 to 500 aa range, expressed in Baculovirus infected Sf9 cells, with >68%, suitable for SDS-PAGE, FuncS.

View Alternative Names

JHDM3B, JMJD2B, KIAA0876, KDM4B, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, [histone H3]-trimethyl-L-lysine(9) demethylase 4B

2 Images
Functional Studies - Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus) (AB198159)
  • FuncS

Unknown

Functional Studies - Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus) (AB198159)

Specific activity of ab198159 was 0.32 pmole/min/μg.

SDS-PAGE - Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus) (AB198159)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human KDM4B / JMJD2B protein (GST tag N-Terminus) (AB198159)

SDS-PAGE analysis of ab198159.

Key facts

Purity

>68% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥2.9 pmol/min/μg.

Assay conditions: 10 μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Accession

O94953

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.55% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB305058

Anti-KDM4B / JMJD2B antibody [EPR25240-51]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry - Anti-KDM4B / JMJD2B antibody [EPR25240-51] (AB305058)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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  • 15
Proteins & Peptides

AB125541

Recombinant Human KDM4A / JHDM3A / JMJD2A protein

SDS-PAGE - Recombinant Human KDM4A / JHDM3A / JMJD2A protein (AB125541)

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Primary Antibodies

AB191433

Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] (AB191433)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Sequence info

[{"sequence":"GSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTALTSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALLEEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKAKSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEES","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":500,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O94953","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed : 16603238, PubMed : 28262558). Plays a critical role in the development of the central nervous system (CNS).

Sequence similarities

Belongs to the JHDM3 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (PubMed : 16603238, PubMed : 28262558). Plays a critical role in the development of the central nervous system (CNS).
See full target information KDM4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102536 PubMed36174675

2022

Intermittent hypoxia enhances the expression of hypoxia inducible factor HIF1A through histone demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chloe-Anne Martinez,Yannasittha Jiramongkol,Neha Bal,Imala Alwis,Polina E Nedoboy,Melissa M J Farnham,Mark D White,Peter A Cistulli,Kristina M Cook
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Product promise

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