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AB198094

Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus)

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(1 Publication)

Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus) is a Human Fragment protein, in the 2 to 372 aa range, expressed in Baculovirus infected Sf9 cells, with >82%, suitable for SDS-PAGE, FuncS.

View Alternative Names

GASC1, JHDM3C, JMJD2C, KIAA0780, KDM4C, Lysine-specific demethylase 4C, Gene amplified in squamous cell carcinoma 1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, [histone H3]-trimethyl-L-lysine(9) demethylase 4C, GASC-1 protein

2 Images
Functional Studies - Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus) (AB198094)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus) (AB198094)

Activity assay using ab198094.

SDS-PAGE - Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus) (AB198094)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM4C / GASC1 / JMJD2C protein (GST tag N-Terminus) (AB198094)

4-20% SDS-PAGE analysis of 0.8 μg ab198094 with Coomassie staining.

Key facts

Purity

>82% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.17 pmole/min/μg.

Assay Conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and ab198094 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.

Accession

Q9H3R0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"EVAEVESPLNPSCKIMTFRPSMEEFREFNKYLAYMESKGAHRAGLAKVIPPKEWKPRQCYDDIDNLLIPAPIQQMVTGQSGLFTQYNIQKKAMTVKEFRQLANSGKYCTPRYLDYEDLERKYWKNLTFVAPIYGADINGSIYDEGVDEWNIARLNTVLDVVEEECGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSVLKKYGIPFDKITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATVRWIDYGKVAKLCTCRKDMVKISMDIFVRKFQPDRYQLWKQGKDIYTIDHTKPTPASTPEVKAWLQRRRKVRKASRS","proteinLength":"Fragment","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9H3R0","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Sequence similarities

Belongs to the JHDM3 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
See full target information KDM4C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102536 PubMed36174675

2022

Intermittent hypoxia enhances the expression of hypoxia inducible factor HIF1A through histone demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chloe-Anne Martinez,Yannasittha Jiramongkol,Neha Bal,Imala Alwis,Polina E Nedoboy,Melissa M J Farnham,Mark D White,Peter A Cistulli,Kristina M Cook
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Product promise

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