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AB196415

Recombinant human KDM4E protein (GST tag N-Terminus)

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Recombinant human KDM4E protein (GST tag N-Terminus) is a Human Fragment protein, in the 2 to 337 aa range, expressed in Baculovirus infected Sf9 cells, with >37%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KDM4DL, KDM5E, KDM4E, Lysine-specific demethylase 4E, KDM4D-like protein, Lysine-specific demethylase 4D-like, [histone H3]-trimethyl-L-lysine(9) demethylase 4E

2 Images
Functional Studies - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

Activity assay of ab196415.

SDS-PAGE - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

10% SDS-PAGE analysis of ab196415 with Coomassie staining.

Key facts

Purity

>37% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity: 0.14 pmole/min/μg.

10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and ab196415 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.

Accession

B2RXH2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KSVHSSPQNTSHTIMTFYPTMEEFADFNTYVAYMESQGAHQAGLAKVIPPKEWKARQMYDDIEDILIATPLQQVTSGQGGVFTQYHKKKKAMRVGQYRRLANSKKYQTPPHQNFADLEQRYWKSHPGNPPIYGADISGSLFEESTKQWNLGHLGTILDLLEQECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKTWYVVPPEHGQHLERLARELFPDISRGCEAFLRHKVALISPTVLKENGIPFNCMTQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYGKMASQCSCGESTVTFSMDPFVRIVQPESYELWKHRQ","proteinLength":"Fragment","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":337,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"B2RXH2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that demethylates trimethylated 'Lys-9' of histone H3 into monomethylated form, thereby playing a central role in histone code (PubMed : 21914792, PubMed : 23451023). Also demethylates trimethylated 'Lys-56' of histone H3 (H3K9me3) to generate the monomethylated state (H3K56me1) (PubMed : 23451023).

Sequence similarities

Belongs to the JHDM3 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that demethylates trimethylated 'Lys-9' of histone H3 into monomethylated form, thereby playing a central role in histone code (PubMed : 21914792, PubMed : 23451023). Also demethylates trimethylated 'Lys-56' of histone H3 (H3K9me3) to generate the monomethylated state (H3K56me1) (PubMed : 23451023).
See full target information KDM4E
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Product promise

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