Recombinant human KDM5A / Jarid1A / RBBP2 protein

Key facts

Purity

>=70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Specific Activity: 0.027 pmole/min/μg

Assay Conditions: 10 μl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 μM α-ketoglutarate, 25 μM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and ab198108 (80 – 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Accession

P29375-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence

M A G V G P G G Y A A E F V P P P E C P V F E P S W E E F T D P L S F I G R I R P L A E K T G I C K I R P P K D W Q P P F A C E V K S F R F T P R V Q R L N E L E A M T R V R L D F L D Q L A K F W E L Q G S T L K I P V V E R K I L D L Y A L S K I V A S K G G F E M V T K E K K W S K V G S R L G Y L P G K G T G S L L K S H Y E R I L Y P Y E L F Q S G V S L M G V Q M P N L D L K E K V E P E V L S T D T Q T S P E P G T R M N I L P K R T R R V K T Q S E S G D V S R N T E L K K L Q I F G A G P K V V G L A M G T K D K E D E V T R R R K V T N R S D A F N M Q M R Q R K G T L S V N F V D L Y V C M F C G R G N N E D K L L L C D G C D D S Y H T F C L I P P L P D V P K G D W R C P K C V A E E C S K P R E A F G F E Q A V R E Y T L Q S F G E M A D N F K S D Y F N M P V H M V P T E L V E K E F W R L V S S I E E D V I V E Y G A D I S S K D F G S G F P V K D G R R K I L P E E E E Y A L S G W N L N N M P V L E Q S V L A H I N V D I S G M K V P W L Y V G M C F S S F C W H I E D H W S Y S I N Y L H W G E P K T W Y G V P S H A A E Q L E E V M R E L A P E L F E S Q P D L L H Q L V T I M N P N V L M E H G V P V Y R T N Q C A G E F V V T F P R A Y H S G F N Q G Y N F A E A V N F C T A D W L P I G R Q C V N H Y R R L R R H C V F S H E E L I F K M A A D P E C L D V G L A A M V C K E L T L M T E E E T R L R E S V V Q M G V L M S E E E V F E L V P D D E R Q C S A C R T T C F L S A L T C S C N P E R L V C L Y H P T D L C P C P M Q K K C L R Y R Y P L E D L P S L L Y G V K V R A Q S Y D T W V S R V T E A L S A N F N H K K D L I E L R V M L E D A E D R K Y P E N D L F R K L R D A V K E A E T C A S V A Q L L L S K K Q K H R Q S P D S G R T R T K L T V E E L K A F V Q Q L F S L P C V I S Q A R Q V K N L L D D V E E F H E R A Q E A M M D E T P D S S K L Q M L I D M G S S L Y V E L P E L P R L K Q E L Q Q A R W L D E V R L T L S D P Q Q V T L D V M K K L I D S G V G L A P H H A V E K A M A E L Q E L L T V S E R W E E K A K V C L Q A R P R H S V A S L E S I V N E A K N I P A F L P N V L S L K E A L Q K A R E W T A K V E A I Q S G S N Y A Y L E Q L E S L S A K G R P I P V R L E A L P Q V E S Q V A A A R A W R E R T G R T F L K K N S S H T L L Q V L S P R T D I G V Y G S G K N R R

Accession

P29375

Protein length

Fragment

Predicted molecular weight

125 kDa

Amino acids

1 to 1090

Nature

Recombinant

Tags

DDDDK tag C-Terminus

Product promise