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AB271566

Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term)

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Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term) is a Human Fragment protein, in the 2 to 1090 aa range, expressed in Baculovirus infected Sf9 cells, with >74%, suitable for SDS-PAGE, FuncS.

View Alternative Names

JARID1A, RBBP2, RBP2, KDM5A, Lysine-specific demethylase 5A, Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Retinoblastoma-binding protein 2, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, RBBP-2

2 Images
Functional Studies - Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term) (AB271566)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term) (AB271566)

Specific activity of ab271566.

SDS-PAGE - Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term) (AB271566)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM5A / Jarid1A / RBBP2 protein (Active) (His N-Term + Avi N-Term + DDDDK C-Term) (AB271566)

SDS-PAGE analysis of 2 μg ab271566.

Key facts

Purity

>74% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus Avi tag N-Terminus DDDDK tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥0.035 pmol/min/μg.

Accession

P29375

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"128 kDa","actualMolecularWeight":null,"aminoAcidEnd":1090,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P29375","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"Avi","terminus":"N-Terminus"},{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed : 18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed : 19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed : 27427228).

Sequence similarities

Belongs to the JARID1 histone demethylase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed : 18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed : 19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed : 27427228).
See full target information KDM5A
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