Recombinant human KDM5B / PLU1 / Jarid1B protein

Key facts

Purity

>=90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Specific Activity: 0.028 pmole/min/μg

Assay Conditions: 10 μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and ab198112 (20 – 100 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Predicted molecular weight

88 kDa

Amino acids

2 to 751

Sequence

E A A T T L H P G P R P A L P L G G P G P L G E F L P P P E C P V F E P S W E E F A D P F A F I H K I R P I A E Q T G I C K V R P P P D W Q P P F A C D V D K L H F T P R I Q R L N E L E A Q T R V K L N F L D Q I A K Y W E L Q G S T L K I P H V E R K I L D L F Q L N K L V A E E G G F A V V C K D R K W T K I A T K M G F A P G K A V G S H I R G H Y E R I L N P Y N L F L S G D S L R C L Q K P N L T T D T K D K E Y K P H D I P Q R Q S V Q P S E T C P P A R R A K R M R A E A M N I K I E P E E T T E A R T H N L R R R M G C P T P K C E N E K E M K S S I K Q E P I E R K D Y I V E N E K E K P K S R S K K A T N A V D L Y V C L L C G S G N D E D R L L L C D G C D D S Y H T F C L I P P L H D V P K G D W R C P K C L A Q E C S K P Q E A F G F E Q A A R D Y T L R T F G E M A D A F K S D Y F N M P V H M V P T E L V E K E F W R L V S T I E E D V T V E Y G A D I A S K E F G S G F P V R D G K I K L S P E E E E Y L D S G W N L N N M P V M E Q S V L A H I T A D I C G M K L P W L Y V G M C F S S F C W H I E D H W S Y S I N Y L H W G E P K T W Y G V P G Y A A E Q L E N V M K K L A P E L F V S Q P D L L H Q L V T I M N P N T L M T H E V P V Y R T N Q C A G E F V I T F P R A Y H S G F N Q G F N F A E A V N F C T V D W L P L G R Q C V E H Y R L L H R Y C V F S H D E M I C K M A S K A D V L D V V V A S T V Q K D M A I M I E D E K A L R E T V R K L G V I D S E R M D F E L L P D D E R Q C V K C K T T C F M S A I S C S C K P G L L V C L H H V K E L C S C P P Y K Y K L R Y R Y T L D D L Y P M M N A L K L R

Accession

Q9UGL1-1

Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Tween, 0.02% Potassium chloride

Notes

Product promise