Recombinant human KDM5B / PLU1 / Jarid1B protein (Active) is a Human Full Length protein, in the 2 to 1544 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus Avi tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
Alternative names
JARID1B, PLU1, RBBP2H1, KDM5B, Lysine-specific demethylase 5B, Cancer/testis antigen 31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, [histone H3]-trimethyl-L-lysine(4) demethylase 5B, CT31, RBP2-H1
Recommended products
Recombinant human KDM5B / PLU1 / Jarid1B protein (Active) is a Human Full Length protein, in the 2 to 1544 aa range, expressed in Baculovirus infected Sf9, with >=75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus Avi tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 0.11 pmol/min/μg.
- Accession
- Q9UGL1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.01% TCEP HCl
Sequence info
Amino acid sequence
- Accession
- Q9UGL1
- Protein length
- Full Length
- Predicted molecular weight
- 179 kDa
- Amino acids
- 2 to 1544
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus, Avi tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
- Sequence similarities
Belongs to the JARID1 histone demethylase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Recombinant human KDM5B / PLU1 / Jarid1B protein (Active) (ab271567)
Specific activity of ab271567.
SDS-PAGE - Recombinant human KDM5B / PLU1 / Jarid1B protein (Active) (ab271567)
SDS-PAGE analysis of 2.3 μg ab271567.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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