Recombinant human KDM6A / UTX protein (Active)

Key facts

Purity

>=58% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Specific Activity: 0.096 pmol/min/μg.

Assay Conditions: 10 μl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1 mM TCEP, 100 μM alpha-ketoglutarate, 25 μM iron, 0.1 mM ascorbic acid, 0.02% Tween-20, 0.5 μM biotinylated peptide substrate, and ab271785 (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Accession

O15550-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.01% TCEP HCl

Sequence info

Amino acid sequence

M K S C G V S L A T A A A A A A A F G D E E K K M A A G K A S G E S E E A S P S L T A E E R E A L G G L D S R L F G F V R F H E D G A R T K A L L G K A V R C Y E S L I L K A E G K V E S D F F C Q L G H F N L L L E D Y P K A L S A Y Q R Y Y S L Q S D Y W K N A A F L Y G L G L V Y F H Y N A F Q W A I K A F Q E V L Y V D P S F C R A K E I H L R L G L M F K V N T D Y E S S L K H F Q L A L V D C N P C T L S N A E I Q F H I A H L Y E T Q R K Y H S A K E A Y E Q L L Q T E N L S A Q V K A T V L Q Q L G W M H H T V D L L G D K A T K E S Y A I Q Y L Q K S L E A D P N S G Q S W Y F L G R C Y S S I G K V Q D A F I S Y R Q S I D K S E A S A D T W C S I G V L Y Q Q Q N Q P M D A L Q A Y I C A V Q L D H G H A A A W M D L G T L Y E S C N Q P Q D A I K C Y L N A T R S K S C S N T S A L A A R I K Y L Q A Q L C N L P Q G S L Q N K T K L L P S I E E A W S L P I P A E L T S R Q G A M N T A Q Q N T S D N W S G G H A V S H P P V Q Q Q A H S W C L T P Q K L Q H L E Q L R A N R N N L N P A Q K L M L E Q L E S Q F V L M Q Q H Q M R P T G V A Q V R S T G I P N G P T A D S S L P T N S V S G Q Q P Q L A L T R V P S V S Q P G V R P A C P G Q P L A N G P F S A G H V P C S T S R T L G S T D T I L I G N N H I T G S G S N G N V P Y L Q R N A L T L P H N R T N L T S S A E E P W K N Q L S N S T Q G L H K G Q S S H S A G P N G E R P L S S T G P S Q H L Q A A G S G I Q N Q N G H P T L P S N S V T Q G A A L N H L S S H T A T S G G Q Q G I T L T K E S K P S G N I L T V P E T S R H T G E T P N S T A S V E G L P N H V H Q M T A D A V C S P S H G D S K S P G L L S S D N P Q L S A L L M G K A N N N V G T G T C D K V N N I H P A V H T K T D N S V A S S P S S A I S T A T P S P K S T E Q T T T N S V T S L N S P H S G L H T I N G E G M E E S Q S P M K T D L L L V N H K P S P Q I I P S M S V S I Y P S S A E V L K A C R N L G K N G L S N S S I L L D K C P P P R P P S S P Y P P L P K D K L N P P T P S I Y L E N K R D A F F P P L H Q F C T N P N N P V T V I R G L A G A L K L D L G L F S T K T L V E A N N E H M V E V R T Q L L Q P A D E N W D P T G T K K I W H C E S N R S H T T I A K Y A Q Y Q A S S F Q E S L R E E N E K R S H H K D H S D S E S T S S D N S G R R R K G P F K T I K F G T N I D L S D D K K W K L Q L H E L T K L P A F V R V V S A G N L L S H V G H T I L G M N T V Q L Y M K V P G S R T P G H Q E N N N F C S V N I N I G P G D C E W F V V P E G Y W G V L N D F C E K N N L N F L M G S W W P N L E D L Y E A N V P V Y R F I Q R P G D L V W I N A G T V H W V Q A I G W C N N I A W N V G P L T A C Q Y K L A V E R Y E W N K L Q S V K S I V P M V H L S W N M A R N I K V S D P K L F E M I K Y C L L R T L K Q C Q T L R E A L I A A G K E I I W H G R T K E E P A H Y C S I C E V E V F D L L F V T N E S N S R K T Y I V H C Q D C A R K T S G N L E N F V V L E Q Y K M E D L M Q V Y D Q F T L A P P L P S A S S

Accession

O15550

Protein length

Full Length

Amino acids

1 to 1401

Nature

Recombinant

Tags

DDDDK tag N-Terminus

Product promise