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AB196420

Recombinant human KDM6B / JMJD3 protein

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Recombinant human KDM6B / JMJD3 protein is a Human Fragment protein, in the 1043 to 1643 aa range, expressed in Baculovirus infected Sf9 cells, with >50%, suitable for SDS-PAGE, FuncS.

View Alternative Names

JMJD3, KIAA0346, KDM6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B

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Functional Studies - Recombinant human KDM6B / JMJD3 protein (AB196420)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KDM6B / JMJD3 protein (AB196420)

Activity assay for ab196420.

SDS-PAGE - Recombinant human KDM6B / JMJD3 protein (AB196420)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM6B / JMJD3 protein (AB196420)

SDS-PAGE of 4μg ab196420 (lane 1). Protein marker (lane 2).
10% gel, coomassie staining.

Key facts

Purity

>50% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity: 0.022 pmole/min/μg.

10μl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JMJD3 (100 – 500 ng) added to the wells. Add antibody against demethylated K27 peptide.

Accession

O15054

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KAKPPTAPAPPSAPAPSAQPTPPSASVPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASRNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLEQYRTEELAQAYDAFTLVRARRARGQRRRALGQAAGTGFGSPAAPFPEPPPAFSPQAPASTSR","proteinLength":"Fragment","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":1643,"aminoAcidStart":1043,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O15054","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed : 17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).

Sequence similarities

Belongs to the UTX family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed : 17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).
See full target information KDM6B
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Complementary Products

Primary Antibodies

AB38113

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Assay Kits

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