Recombinant Human ketohexokinase protein is a Human Full Length protein, in the 1 to 298 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M E E K Q I L C V G L V V L D V I S L V D K Y P K E D S E I R C L S Q R W Q R G G N A S N S C T I L S L L G A P C A F M G S M A P G H V A D F V L D D L R R Y S V D L R Y T V F Q T T G S V P I A T V I I N E A S G S R T I L Y Y D R S L P D V S A T D F E K V D L T Q F K W I H I E G R N A S E Q V K M L Q R I D A H N T R Q P P E Q K I R V S V E V E K P R E E L F Q L F G Y G D V V F V S K D V A K H L G F Q S A E E A L R G L Y G R V R K G A V L V C A W A E E G A D A L G P D G K L L H S D A F P P P R V V D T L G A G D T F N A S V I F S L S Q G R S V Q E A L R F G C Q V A G K K C G L Q G F D G I V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Alternative names
Ketohexokinase, Hepatic fructokinase, KHK
Recommended products
Recombinant Human ketohexokinase protein is a Human Full Length protein, in the 1 to 298 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P50053-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M E E K Q I L C V G L V V L D V I S L V D K Y P K E D S E I R C L S Q R W Q R G G N A S N S C T I L S L L G A P C A F M G S M A P G H V A D F V L D D L R R Y S V D L R Y T V F Q T T G S V P I A T V I I N E A S G S R T I L Y Y D R S L P D V S A T D F E K V D L T Q F K W I H I E G R N A S E Q V K M L Q R I D A H N T R Q P P E Q K I R V S V E V E K P R E E L F Q L F G Y G D V V F V S K D V A K H L G F Q S A E E A L R G L Y G R V R K G A V L V C A W A E E G A D A L G P D G K L L H S D A F P P P R V V D T L G A G D T F N A S V I F S L S Q G R S V Q E A L R F G C Q V A G K K C G L Q G F D G I V
- Accession
- P50053
- Protein length
- Full Length
- Predicted molecular weight
- 32.7 kDa
- Amino acids
- 1 to 298
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab95395 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
- Sequence similarities
Belongs to the carbohydrate kinase PfkB family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ketohexokinase also known as KHK or fructokinase is an enzyme that catalyzes the phosphorylation of fructose to fructose-1-phosphate utilizing ATP in the process. This enzyme has two isoforms: KHK-C and KHK-A with the cytosolic form (KHK-C) being approximately 35 kDa in mass. KHK is expressed at high levels in the liver kidney cortex and small intestine showing its importance for fructose metabolism in these tissues. The liver in particular exhibits extensive expression which facilitates the initial step in fructose metabolism.
- Biological function summary
Ketohexokinase plays a significant role in the initial stage of fructose metabolism by converting fructose to fructose-1-phosphate. This reaction is a part of the fructose metabolic pathway enabling the use of fructose as an energy source especially in hepatocytes. KHK operates as a monomer and does not form part of any larger protein complex. The enzyme importantly controls the rate of fructose utilization preventing accumulation of free fructose which could lead to metabolic issues.
- Pathways
Ketohexokinase belongs to the fructose metabolism pathway which is a subset of the larger carbohydrate metabolism. This enzyme's product fructose-1-phosphate subsequently interacts with aldolase B which is involved in further steps of the glycolysis and gluconeogenesis pathways. Besides aldolase B ketohexokinase's activity influences the balance between fructose and glucose metabolism connecting fructolysis with glycolytic and gluconeogenic pathways.
- Associated diseases and disorders
Dysfunction of ketohexokinase has been linked to hereditary fructose intolerance a condition resulting from a deficiency of aldolase B where the accumulation of fructose-1-phosphate can cause liver damage and hypoglycemia. KHK has also been implicated in metabolic syndrome where excessive fructose consumption exacerbates conditions like insulin resistance and fatty liver disease. The relationship of KHK with metabolic syndrome highlights its interaction with insulin pathways which involves proteins such as insulin receptor substrates that are affected by altered fructose metabolism.
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1 product image
SDS-PAGE - Recombinant Human ketohexokinase protein (ab95395)
15% SDS-PAGE showing ab95395 at approximately 32.7kDa (3μg).
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