Recombinant human KGF/FGF-7 protein (Active) is a Human Full Length protein, in the 32 to 194 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
C N D M T P E Q M A T N V N C S S P E R H T R S Y D Y M E G G D I R V R R L F C R T Q W Y L R I D K R G K V K G T Q E M K N N Y N I M E I R T V A V G I V A I K G V E S E F Y L A M N K E G K L Y A K K E C N E D C N F K E L I L E N H Y N T Y A S A K W T H N G G E M F V A L N Q K G I P V R G K K T K K E Q K T A H F L P M A I T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
Alternative names
KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7
Recommended products
Recombinant human KGF/FGF-7 protein (Active) is a Human Full Length protein, in the 32 to 194 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of 4MBr-5 cells. ED50 is ≤11.53 ng/ml, corresponding to a specific activity of 8.7 x 104 units/mg.
- Accession
- P21781-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- C N D M T P E Q M A T N V N C S S P E R H T R S Y D Y M E G G D I R V R R L F C R T Q W Y L R I D K R G K V K G T Q E M K N N Y N I M E I R T V A V G I V A I K G V E S E F Y L A M N K E G K L Y A K K E C N E D C N F K E L I L E N H Y N T Y A S A K W T H N G G E M F V A L N Q K G I P V R G K K T K K E Q K T A H F L P M A I T
- Accession
- P21781
- Protein length
- Full Length
- Predicted molecular weight
- 18.94 kDa
- Actual molecular weight
- 18.94 kDa
- Amino acids
- 32 to 194
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Equal or greater than 95% by HPLC
General info
- Function
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant human KGF/FGF-7 protein (Active) (ab283451)
SDS-PAGE analysis of ab283451.
Mass Spectrometry - Recombinant human KGF/FGF-7 protein (Active) (ab283451)
Predicted MW is 18940.76 Da. (+/- 10 Da by ESI-TOF). Observed MW is 18942.12 Da. Additional masses at 19510.56 are due to residual O-GalNAc-GloNAc-Gal.
HPLC - Recombinant human KGF/FGF-7 protein (Active) (ab283451)
HPLC analysis of ab283451
Functional Studies - Recombinant human KGF/FGF-7 protein (Active) (ab283451)
Fully biologically active determined by dose dependent proliferation of 4MBr-5 cells. ED50 is ≤11.53 ng/ml, corresponding to a specific activity of 8.7 x 104 units/mg.
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