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AB283451

Recombinant human KGF/FGF-7 protein (Active)

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Recombinant human KGF/FGF-7 protein (Active) is a Human Full Length protein, in the 32 to 194 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7

4 Images
Functional Studies - Recombinant human KGF/FGF-7 protein (Active) (AB283451)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KGF/FGF-7 protein (Active) (AB283451)

Fully biologically active determined by dose dependent proliferation of 4MBr-5 cells. ED50 is ≤11.53 ng/ml, corresponding to a specific activity of 8.7 x 104 units/mg.

Mass Spectrometry - Recombinant human KGF/FGF-7 protein (Active) (AB283451)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human KGF/FGF-7 protein (Active) (AB283451)

Predicted MW is 18940.76 Da. (+/- 10 Da by ESI-TOF). Observed MW is 18942.12 Da. Additional masses at 19510.56 are due to residual O-GalNAc-GloNAc-Gal.

SDS-PAGE - Recombinant human KGF/FGF-7 protein (Active) (AB283451)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KGF/FGF-7 protein (Active) (AB283451)

SDS-PAGE analysis of ab283451.

HPLC - Recombinant human KGF/FGF-7 protein (Active) (AB283451)
  • HPLC

Supplier Data

HPLC - Recombinant human KGF/FGF-7 protein (Active) (AB283451)

HPLC analysis of ab283451

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, Cell Culture, HPLC, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent proliferation of 4MBr-5 cells. ED50 is ≤11.53 ng/ml, corresponding to a specific activity of 8.7 x 104 units/mg.

Accession

P21781

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT","proteinLength":"Full Length","predictedMolecularWeight":"18.94 kDa","actualMolecularWeight":"18.94 kDa","aminoAcidEnd":194,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P21781","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

Equal or greater than 95% by HPLC

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General info

Function

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

Sequence similarities

Belongs to the heparin-binding growth factors family.

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Product protocols

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Target data

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
See full target information FGF7
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Product promise

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