Recombinant Human KLF12 protein (denatured) is a Human Full Length protein, in the 1 to 402 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M N I H M K R K T I K N I N T F E N R M L M L D G M P A V R V K T E L L E S E Q G S P N V H N Y P D M E A V P L L L N N V K G E P P E D S L S V D H F Q T Q T E P V D L S I N K A R T S P T A V S S S P V S M T A S A S S P S S T S T S S S S S S R L A S S P T V I T S V S S A S S S S T V L T P G P L V A S A S G V G G Q Q F L H I I H P V P P S S P M N L Q S N K L S H V H R I P V V V Q S V P V V Y T A V R S P G N V N N T I V V P L L E D G R G H G K A Q M D P R G L S P R Q S K S D S D D D D L P N V T L D S V N E T G S T A L S I A R A V Q E V H P S P V S R V R G N R M N N Q K F P C S I S P F S I E S T R R Q R R S E S P D S R K R R I H R C D F E G C N K V Y T K S S H L K A H R R T H T G E K P Y K C T W E G C T W K F A R S D E L T R H Y R K H T G V K P F K C A D C D R S F S R S D H L A L H R R R H M L V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.
Alternative names
AP2REP, HSPC122, KLF12, Krueppel-like factor 12, Transcriptional repressor AP-2rep
Recommended products
Recombinant Human KLF12 protein (denatured) is a Human Full Length protein, in the 1 to 402 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9Y4X4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M N I H M K R K T I K N I N T F E N R M L M L D G M P A V R V K T E L L E S E Q G S P N V H N Y P D M E A V P L L L N N V K G E P P E D S L S V D H F Q T Q T E P V D L S I N K A R T S P T A V S S S P V S M T A S A S S P S S T S T S S S S S S R L A S S P T V I T S V S S A S S S S T V L T P G P L V A S A S G V G G Q Q F L H I I H P V P P S S P M N L Q S N K L S H V H R I P V V V Q S V P V V Y T A V R S P G N V N N T I V V P L L E D G R G H G K A Q M D P R G L S P R Q S K S D S D D D D L P N V T L D S V N E T G S T A L S I A R A V Q E V H P S P V S R V R G N R M N N Q K F P C S I S P F S I E S T R R Q R R S E S P D S R K R R I H R C D F E G C N K V Y T K S S H L K A H R R T H T G E K P Y K C T W E G C T W K F A R S D E L T R H Y R K H T G V K P F K C A D C D R S F S R S D H L A L H R R R H M L V
- Accession
- Q9Y4X4
- Protein length
- Full Length
- Predicted molecular weight
- 46.6 kDa
- Amino acids
- 1 to 402
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab187457 was purified using conventional chromatography.
General info
- Function
Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.
- Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF12 also known as Krüppel-like factor 12 is a transcription factor with a mass of approximately 37 kDa. This protein is expressed in diverse tissues including the liver heart and brain. KLF12 modulates gene expression by binding to specific DNA sequences with its zinc finger domains. The factor plays an important role in transcription regulation by interacting with promoter regions of target genes.
- Biological function summary
KLF12 influences cell differentiation proliferation and apoptosis. It operates as part of transcriptional complexes often interacting with co-regulators such as histone deacetylases. The protein controls the expression of critical genes involved in cellular growth and maintenance exerting broad impacts on cellular phenotype. KLF12's interaction with co-repressors alters chromatin structure influencing transcriptional landscapes.
- Pathways
KLF12 regulates the Wnt signaling pathway which is significant for controlling cell fate and migration. KLF12 interacts with components like beta-catenin altering the pathway's output and affecting cellular responses to developmental signals. Additionally KLF12 is linked to the TGF-beta signaling pathway influencing related proteins and impacting cellular processes such as proliferation and differentiation.
- Associated diseases and disorders
KLF12 shows strong associations with cancer particularly colorectal cancer. It interacts with proteins like E-cadherin affecting the epithelial-to-mesenchymal transition an important step in cancer metastasis. Furthermore KLF12 also connects to neurodegenerative disorders influencing factors linked to neural integrity and function underlining its potential role in pathological processes.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human KLF12 protein (denatured) (ab187457)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com