Recombinant Human KLF15 protein is a Human Full Length protein, in the 1 to 416 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M V D H L L P V D E N F S S P K C P V G Y L G D R L V G R R A Y H M L P S P V S E D D S D A S S P C S C S S P D S Q A L C S C Y G G G L G T E S Q D S I L D F L L S Q A T L G S G G G S G S S I G A S S G P V A W G P W R R A A A P V K G E H F C L P E F P L G D P D D V P R P F Q P T L E E I E E F L E E N M E P G V K E V P E G N S K D L D A C S Q L S A G P H K S H L H P G S S G R E R C S P P P G G A S A G G A Q G P G G G P T P D G P I P V L L Q I Q P V P V K Q E S G T G P A S P G Q A P E N V K V A Q L L V N I Q G Q T F A L V P Q V V P S S N L N L P S K F V R I A P V P I A A K P V G S G P L G P G P A G L L M G Q K F P K N P A A E L I K M H K C T F P G C S K M Y T K S S H L K A H L R R H T G E K P F A C T W P G C G W R F S R S D E L S R H R R S H S G V K P Y Q C P V C E K K F A R S D H L S K H I K V H R F P R S S R S V R S V N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Target data
Function
Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
Alternative names
KKLF, KLF15, Krueppel-like factor 15, Kidney-enriched krueppel-like factor
Recommended products
Recombinant Human KLF15 protein is a Human Full Length protein, in the 1 to 416 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9UIH9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M V D H L L P V D E N F S S P K C P V G Y L G D R L V G R R A Y H M L P S P V S E D D S D A S S P C S C S S P D S Q A L C S C Y G G G L G T E S Q D S I L D F L L S Q A T L G S G G G S G S S I G A S S G P V A W G P W R R A A A P V K G E H F C L P E F P L G D P D D V P R P F Q P T L E E I E E F L E E N M E P G V K E V P E G N S K D L D A C S Q L S A G P H K S H L H P G S S G R E R C S P P P G G A S A G G A Q G P G G G P T P D G P I P V L L Q I Q P V P V K Q E S G T G P A S P G Q A P E N V K V A Q L L V N I Q G Q T F A L V P Q V V P S S N L N L P S K F V R I A P V P I A A K P V G S G P L G P G P A G L L M G Q K F P K N P A A E L I K M H K C T F P G C S K M Y T K S S H L K A H L R R H T G E K P F A C T W P G C G W R F S R S D E L S R H R R S H S G V K P Y Q C P V C E K K F A R S D H L S K H I K V H R F P R S S R S V R S V N
- Accession
- Q9UIH9
- Protein length
- Full Length
- Predicted molecular weight
- 70.4 kDa
- Amino acids
- 1 to 416
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
- Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF15 also known as Kruppel-like factor 15 works mechanically as a transcription factor. The KLF15 protein with a molecular mass of approximately 44 kDa functions by regulating the expression of genes involved in metabolism and cell differentiation. It is expressed in various tissues including the heart liver and skeletal muscle. This expression suggests that the KLF-15 protein plays a significant role in maintaining tissue-specific functions.
- Biological function summary
KLF15 influences several cellular processes by regulating gene expression. It is part of a transcription factor complex that modulates glucose metabolism lipid metabolism and muscle differentiation. The KLF-15 protein contributes to maintaining homeostasis by upregulating genes responsible for gluconeogenesis and lipid usage which is essential in adapting to fasting states. Additionally it impacts muscle cell differentiation by influencing the expression of specific myogenic factors.
- Pathways
KLF15 is significant in metabolic and myogenic pathways. It upregulates genes involved in the gluconeogenesis pathway and fatty acid oxidation collaborating with proteins such as PGC-1α. Additionally within myogenic pathways KLF-15 protein interacts with myogenic regulatory factors contributing to muscle differentiation. These interactions highlight its importance in metabolic and muscle function pathways and highlight its regulatory capacity across different biological processes.
- Associated diseases and disorders
KLF15 has important roles. Alterations in KLF-15 protein levels link to metabolic diseases such as diabetes and cardiac hypertrophy. In diabetes it affects glucose metabolism and insulin sensitivity with connections to proteins like FOXO1. In cardiac hypertrophy KLF-15 influences structural remodeling of the heart potentially interacting with hypertrophy-associated factors. This connection to diseases emphasizes its potential as a therapeutic target for related disorders.
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1 product image
SDS-PAGE - Recombinant Human KLF15 protein (ab131876)
12.5% SDS-PAGE analysis of ab131876 stained with Coomassie Blue.
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