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AB169919

Recombinant Human KLF17 protein

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Recombinant Human KLF17 protein is a Human Full Length protein, in the 2 to 389 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ZNF393, KLF17, Krueppel-like factor 17, Zinc finger protein 393

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q5JT82

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris-HCl buffer

storage-buffer

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Complementary Products

Assay Kits

AB197751

Mouse E-Cadherin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

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Primary Antibodies

AB231303

Anti-E Cadherin antibody [4A2]

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-E Cadherin antibody [4A2] (AB231303)

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  • 4
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFYGRPQAEMEQEAGELSRWQAAHQAAQDNENSAPILNMSSSSGSSGVHTSWNQGLPTIQHFPHSAEMLGSPLVSVEAPGQNVNEGGPQFSMPLPERGMSYCPQATLTPSRMIYCQRMSPPQQEMTIFSGPQLMPVGEPNIPRVARPFGGNLRMPPSGLPVSASTGIPIMSHTGNPPVPYPGLSTVPSDETLLGPTVPSTEAQAVLPSMAQMLPPQDAHDLGMPPAESQSLLVLGSQDSLVSQPDSQEGPFLPEQPGPAPQTVEKNSRPQEGTGRRGSSEARPYCCNYENCGKAYTKRSHLVSHQRKHTGERPYSCNWESCSWSFFRSDELRRHMRVHTRYRPYKCDQCSREFMRSDHLKQHQKTHRPGPSDPQANNNNGEQDSPPAAGP","proteinLength":"Full Length","predictedMolecularWeight":"45.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":389,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q5JT82","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF17 also known as Krüppel-like factor 17 is a zinc-finger transcription factor with a mass of approximately 35 kDa. This protein plays a role in DNA-binding and regulation of transcription. It regulates the expression of target genes by binding to GC-rich regions. Researchers have observed KLF17 expression in various tissues with notable levels in the reproductive organs such as the ovary and testis as well as in embryonic tissues which indicates its involvement in reproductive biology and development.
Biological function summary

KLF17 influences cellular processes by modulating the expression of genes involved in cell differentiation and proliferation. It functions primarily as a transcriptional repressor acting independently and not as part of a larger multiprotein complex. Its regulatory role affects key cellular functions contributing to the control of epithelial-mesenchymal transition (EMT) which is critical during embryogenesis and cancer metastasis.

Pathways

KLF17 plays a significant role in the TGF-β signaling pathway which is important in numerous cellular functions such as growth and differentiation. KLF17 interacts with other transcription factors like SNAIL and TWIST influencing pathways that control EMT and metastasis. This regulation within TGF-β signaling highlights KLF17's role in maintaining cellular architecture and regulating processes critical to tissue development and disease progression.

KLF17 shows a connection to cancer and fibrosis. Researchers have identified its involvement in the suppression of metastasis in several cancers including breast cancer by interacting with proteins like E-cadherin which maintain cell adhesion. In fibrosis alterations in KLF17 expression can disrupt normal tissue repair processes exacerbating the progression of fibrotic diseases by interacting with mediators involved in the TGF-β signaling pathway.
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Specifications

Form

Liquid

Additional notes

ab169919 was expressed in E.coli as inclusion bodies. The final product was refolded and chromatographically purified.

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General info

Function

Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development (By similarity).

Sequence similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development (By similarity).
See full target information KLF17
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Product promise

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