JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB167832

Recombinant Human KLF3 protein (denatured)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human KLF3 protein (denatured) is a Human protein, in the 1 to 345 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

BKLF, KLF3, Krueppel-like factor 3, Basic krueppel-like factor, CACCC-box-binding protein BKLF, TEF-2

1 Images
SDS-PAGE - Recombinant Human KLF3 protein (denatured) (AB167832)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KLF3 protein (denatured) (AB167832)

15% SDS-PAGE analysis of ab167832 (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P57682

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMLMFDPVPVKQEAMDPVSVSYPSNYMESMKPNKYGVIYSTPLPEKFFQTPEGLSHGIQMEPVDLTVNKRSSPPSAGNSPSSLKFPSSHRRASPGLSMPSSSPPIKKYSPPSPGVQPFGVPLSMPPVMAAALSRHGIRSPGILPVIQPVVVQPVPFMYTSHLQQPLMVSLSEEMENSSSSMQVPVIESYEKPISQKKIKIEPGIEPQRTDYYPEEMSPPLMNSVSPPQALLQENHPSVIVQPGKRPLPVESPDTQRKRRIHRCDYDGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKFARSDELTRHFRKHTGIKPFQCPDCDRSFSRSDHLALHRKRHMLV","proteinLength":null,"predictedMolecularWeight":"41.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":345,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P57682","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF3 also known as BKLF (Basic Krüppel-like Factor) is a transcription factor weighing approximately 40 kDa. It regulates gene expression by binding to specific DNA sequences generally influencing various cellular processes. KLF3 is expressed in diverse tissues such as hematopoietic cells and adipose tissues among others. Its presence is observed in both normal physiological conditions and under certain stresses indicating a broader regulatory role.
Biological function summary

KLF3 acts as a major regulator of cell differentiation and proliferation. It functions as part of a transcriptional complex interacting with co-factors to modulate gene networks. Through its zinc finger domains KLF3 binds to DNA and influences the transcription of genes involved in erythropoiesis the process that produces red blood cells and adipogenesis the formation of fat cells. These biological roles suggest that KLF3 maintains homeostasis in tissue development and maturation.

Pathways

Multiple cellular events involve KLF3. It significantly contributes to the TGF-beta signaling pathway and the erythropoiesis pathway. Within these pathways KLF3 interacts with other proteins like TGF-beta receptors and other transcription factors like GATA1. This interaction helps drive transcriptional changes necessary for these pathways' completion indicating KLF3's integral role.

KLF3 plays a part in several conditions including obesity and anemia. In obesity alteration in KLF3 expression affects adipogenesis impacting fat cell formation and accumulation. In anemia KLF3 dysregulation leads to improper erythropoiesis due to its interaction with proteins like GATA1 and EPOR (erythropoietin receptor). These associations underline KLF3's potential implications in managing metabolic and blood disorders.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity).

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Post-translational modifications

Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site (By similarity).

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity).
See full target information KLF3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB154531

Anti-KLF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (AB154531)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com