Recombinant Human KLHDC8B protein (denatured) is a Human Full Length protein, in the 1 to 354 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S A G G G R A F A W Q V F P P M P T C R V Y G T V A H Q D G H L L V L G G C G R A G L P L D T A E T L D M A S H T W L A L A P L P T A R A G A A A V V L G K Q V L V V G G V D E V Q S P V A A V E A F L M D E G R W E R R A T L P Q A A M G V A T V E R D G M V Y A L G G M G P D T A P Q A Q V R V Y E P R R D C W L S L P S M P T P C Y G A S T F L H G N K I Y V L G G R Q G K L P V T A F E A F D L E A R T W T R H P S L P S R R A F A G C A M A E G S V F S L G G L Q Q P G P H N F Y S R P H F V N T V E M F D L E H G S W T K L P R S L R M R D K R A D F V V G S L G G H I V A I G G L G N Q P C P L G S V E S F S L A R R R W E A L P A M P T A R C S C S S L Q A G P R L F V I G G V A Q G P S Q A V E A L C L R D G V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis (PubMed:20107318). Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells (PubMed:22988245, PubMed:23713010).
Alternative names
FP17659, KLHDC8B, Kelch domain-containing protein 8B
Recommended products
Recombinant Human KLHDC8B protein (denatured) is a Human Full Length protein, in the 1 to 354 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8IXV7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S A G G G R A F A W Q V F P P M P T C R V Y G T V A H Q D G H L L V L G G C G R A G L P L D T A E T L D M A S H T W L A L A P L P T A R A G A A A V V L G K Q V L V V G G V D E V Q S P V A A V E A F L M D E G R W E R R A T L P Q A A M G V A T V E R D G M V Y A L G G M G P D T A P Q A Q V R V Y E P R R D C W L S L P S M P T P C Y G A S T F L H G N K I Y V L G G R Q G K L P V T A F E A F D L E A R T W T R H P S L P S R R A F A G C A M A E G S V F S L G G L Q Q P G P H N F Y S R P H F V N T V E M F D L E H G S W T K L P R S L R M R D K R A D F V V G S L G G H I V A I G G L G N Q P C P L G S V E S F S L A R R R W E A L P A M P T A R C S C S S L Q A G P R L F V I G G V A Q G P S Q A V E A L C L R D G V
- Accession
- Q8IXV7
- Protein length
- Full Length
- Predicted molecular weight
- 40.1 kDa
- Amino acids
- 1 to 354
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis (PubMed:20107318). Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells (PubMed:22988245, PubMed:23713010).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLHDC8B also known as Kelch domain-containing protein 8B is a mechanical component in cellular functions. This protein has a mass of approximately 32 kDa. It is expressed in various tissues with particular presence in the brain and reproductive organs. KLHDC8B contains a Kelch motif which is known for facilitating protein-protein interactions making it essential for various intracellular processes.
- Biological function summary
KLHDC8B plays a role in maintaining cell shape and integrity through its interactions with other cytoskeletal proteins. It forms part of protein complexes that modulate actin filament dynamics. Besides structural functions KLHDC8B participates in processes related to cell movement and division. It remains active in regions with intense cytoskeletal activity reflecting its functional importance.
- Pathways
KLHDC8B integrates into the Wnt signaling and cytoskeletal regulation pathways. Through the Wnt pathway it potentially associates with proteins such as β-catenin which impacts cell proliferation and migration. In cytoskeletal regulation it coordinates with actin and tubulin influencing cellular architecture and transport mechanisms essential for cellular activities.
- Associated diseases and disorders
KLHDC8B has connections to cancers including certain brain tumors and reproductive disorders like infertility. Alterations in KLHDC8B expression can impact cytoskeletal arrangement leading to aberrant cellular growth and division seen in these diseases. Through these associations KLHDC8B links with other proteins such as p53 known for tumor suppressive functions and pathways in cellular stress responses.
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1 product image
SDS-PAGE - Recombinant Human KLHDC8B protein (denatured) (ab187414)
15% SDS-PAGE analysis of ab187414 (3μg).
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