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Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa.

- Save time and ensure accurate results - use our Klotho protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

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Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Tags
Tag free
Applications
HPLC, SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

E P G D G A Q T W A R F S R P P A P E A A G L F Q G T F P D G F L W A V G S A A Y Q T E G G W Q Q H G K G A S I W D T F T H H P L A P P G D S R N A S L P L G A P S P L Q P A T G D V A S D S Y N N V F R D T E A L R E L G V T H Y R F S I S W A R V L P N G S A G V P N R E G L R Y Y R R L L E R L R E L G V Q P V V T L Y H W D L P Q R L Q D A Y G G W A N R A L A D H F R D Y A E L C F R H F G G Q V K Y W I T I D N P Y V V A W H G Y A T G R L A P G I R G S P R L G Y L V A H N L L L A H A K V W H L Y N T S F R P T Q G G Q V S I A L S S H W I N P R R M T D H S I K E C Q K S L D F V L G W F A K P V F I D G D Y P E S M K N N L S S I L P D F T E S E K K F I K G T A D F F A L C F G P T L S F Q L L D P H M K F R Q L E S P N L R Q L L S W I D L E F N H P Q I F I V E N G W F V S G T T K R D D A K Y M Y Y L K K F I M E T L K A I K L D G V D V I G Y T A W S L M D G F E W H R G Y S I R R G L F Y V D F L S Q D K M L L P K S S A L F Y Q K L I E K N G F P P L P E N Q P L E G T F P C D F A W G V V D N Y I Q V S Q L T K P I S S L T K P Y H

Reactivity data

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

ab84072 is a glycoprotein of 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions. ab84072 has a calculated molecular weight of 58.6 kDa.

Application
FuncS
Reactivity
Reacts
Dilution info
0.5-2 µg/mL
Notes

-

Target data

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Alternative names

Recommended products

Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa.

- Save time and ensure accurate results - use our Klotho protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Applications
HPLC, SDS-PAGE, FuncS
Biological activity
Determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 cells.

ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.

Accession
Q9UEF7-1
Animal free
No
Species
Human
Reconstitution
Reconstitute in water
Concentration
Loading...

Sequence info

Amino acid sequence

E P G D G A Q T W A R F S R P P A P E A A G L F Q G T F P D G F L W A V G S A A Y Q T E G G W Q Q H G K G A S I W D T F T H H P L A P P G D S R N A S L P L G A P S P L Q P A T G D V A S D S Y N N V F R D T E A L R E L G V T H Y R F S I S W A R V L P N G S A G V P N R E G L R Y Y R R L L E R L R E L G V Q P V V T L Y H W D L P Q R L Q D A Y G G W A N R A L A D H F R D Y A E L C F R H F G G Q V K Y W I T I D N P Y V V A W H G Y A T G R L A P G I R G S P R L G Y L V A H N L L L A H A K V W H L Y N T S F R P T Q G G Q V S I A L S S H W I N P R R M T D H S I K E C Q K S L D F V L G W F A K P V F I D G D Y P E S M K N N L S S I L P D F T E S E K K F I K G T A D F F A L C F G P T L S F Q L L D P H M K F R Q L E S P N L R Q L L S W I D L E F N H P Q I F I V E N G W F V S G T T K R D D A K Y M Y Y L K K F I M E T L K A I K L D G V D V I G Y T A W S L M D G F E W H R G Y S I R R G L F Y V D F L S Q D K M L L P K S S A L F Y Q K L I E K N G F P P L P E N Q P L E G T F P C D F A W G V V D N Y I Q V S Q L T K P I S S L T K P Y H
Accession
Q9UEF7
Protein length
Fragment
Predicted molecular weight
58.6 kDa
Amino acids
34 to 549
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

> 98% HPLC analyses.

General info

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Post-translational modifications

N-glycosylated.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Ensure the validity of your result using our bioactive recombinant human Klotho (KL) protein ab84072 as a positive control in SDS-PAGE.
The rKlotho ab84072 predicted MW is 58.6 kDa and migrates at an apparent molecular weight of 65- 70 kDa by SDS-PAGE analysis under reducing conditions.

ab84072 endotoxin level is less than 0.1 ng per µg (1EU/µg)


Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.

Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Associated diseases and disorders

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.

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