Recombinant human Klotho protein (Active)

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

HPLC, SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 cells.

ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.

Predicted molecular weight

58.6 kDa

Amino acids

34 to 549

Sequence

E P G D G A Q T W A R F S R P P A P E A A G L F Q G T F P D G F L W A V G S A A Y Q T E G G W Q Q H G K G A S I W D T F T H H P L A P P G D S R N A S L P L G A P S P L Q P A T G D V A S D S Y N N V F R D T E A L R E L G V T H Y R F S I S W A R V L P N G S A G V P N R E G L R Y Y R R L L E R L R E L G V Q P V V T L Y H W D L P Q R L Q D A Y G G W A N R A L A D H F R D Y A E L C F R H F G G Q V K Y W I T I D N P Y V V A W H G Y A T G R L A P G I R G S P R L G Y L V A H N L L L A H A K V W H L Y N T S F R P T Q G G Q V S I A L S S H W I N P R R M T D H S I K E C Q K S L D F V L G W F A K P V F I D G D Y P E S M K N N L S S I L P D F T E S E K K F I K G T A D F F A L C F G P T L S F Q L L D P H M K F R Q L E S P N L R Q L L S W I D L E F N H P Q I F I V E N G W F V S G T T K R D D A K Y M Y Y L K K F I M E T L K A I K L D G V D V I G Y T A W S L M D G F E W H R G Y S I R R G L F Y V D F L S Q D K M L L P K S S A L F Y Q K L I E K N G F P P L P E N Q P L E G T F P C D F A W G V V D N Y I Q V S Q L T K P I S S L T K P Y H

Accession

Q9UEF7-1

Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage. Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Concentration

Notes

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