Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa.
- Save time and ensure accurate results - use our Klotho protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
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| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes ab84072 is a glycoprotein of 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions. ab84072 has a calculated molecular weight of 58.6 kDa. |
Application FuncS | Reactivity Reacts | Dilution info 0.5-2 µg/mL | Notes - |
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Klotho, KL
Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa.
- Save time and ensure accurate results - use our Klotho protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.
> 98% HPLC analyses.
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
N-glycosylated.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Ensure the validity of your result using our bioactive recombinant human Klotho (KL) protein ab84072 as a positive control in SDS-PAGE.
The rKlotho ab84072 predicted MW is 58.6 kDa and migrates at an apparent molecular weight of 65- 70 kDa by SDS-PAGE analysis under reducing conditions.
ab84072 endotoxin level is less than 0.1 ng per µg (1EU/µg)
Check out our protein gel staining guide for SDS-PAGE here
Check out of western blot protocol for more information here
Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.
Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.
The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.
Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.
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