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AB84072

Recombinant human Klotho protein (Active)

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(9 Publications)

Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa.

- Save time and ensure accurate results - use our Klotho protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

View Alternative Names

Klotho, KL

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

HPLC, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 cells.

ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.

Accession

Q9UEF7

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab84072 is a glycoprotein of 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions. ab84072 has a calculated molecular weight of 58.6 kDa.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.5-2 µg/mL", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human Klotho (KL) protein ab84072 as a positive control in SDS-PAGE.
The rKlotho ab84072 predicted MW is 58.6 kDa and migrates at an apparent molecular weight of 65- 70 kDa by SDS-PAGE analysis under reducing conditions.

ab84072 endotoxin level is less than 0.1 ng per μg (1EU/µg)


Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here
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Sequence info

[{"sequence":"EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVSQLTKPISSLTKPYH","proteinLength":"Fragment","predictedMolecularWeight":"58.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":549,"aminoAcidStart":34,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"Q9UEF7","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.
Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.
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Specifications

Form

Lyophilized

Additional notes

> 98% HPLC analyses.

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General info

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
See full target information KL
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease 16:1626-1638 PubMed39012668

2024

SARS-CoV-2 S Protein Reduces Cytoprotective Defenses and Promotes Human Endothelial Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Villacampa,Licia Shamoon,Inés Valencia,Cristina Morales,Sofía Figueiras,Fernando de la Cuesta,Dolores Sánchez-Niño,Guillermo Díaz-Araya,Isabel Sánchez-Pérez,Óscar Lorenzo,Carlos Félix Sánchez-Ferrer,Concepción Peiró

Molecular neurobiology 61:2336-2356 PubMed37875707

2023

STAT4-Mediated Klotho Up-Regulation Contributes to the Brain Ischemic Tolerance by Cerebral Ischemic Preconditioning via Inhibiting Neuronal Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi-Yun Liu,Ling-Yan Zhang,Xiao-Yu Wang,Shi-Chao Li,Yu-Yan Hu,Jing-Ge Zhang,Xiao-Hui Xian,Wen-Bin Li,Min Zhang

International journal of medical sciences 20:810-817 PubMed37213666

2023

Recombinant Klotho attenuates IFNγ receptor signaling and SAMHD1 expression through blocking NF-κB translocation in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kuen-Daw Tsai,Yi-Chao Lee,Bo-Yu Chen,Li-Syuan Wu,Shan-Yuan Liang,Ming-Yuan Liu,Yu-Wen Hung,Hui-Ling Hsu,Pei-Qi Chen,Jia-Ching Shieh,Yi-Ju Lee,Ting-Hui Lin

Aging and disease 13:284-297 PubMed35111374

2022

Pharmacological Blockade of NLRP3 Inflammasome/IL-1β-Positive Loop Mitigates Endothelial Cell Senescence and Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Romero,Pilar Dongil,Inés Valencia,Susana Vallejo,Álvaro San Hipólito-Luengo,Guillermo Díaz-Araya,José L Bartha,María M González-Arlanzón,Fernando Rivilla,Fernando de la Cuesta,Carlos F Sánchez-Ferrer,Concepción Peiró

International journal of molecular sciences 22: PubMed34360633

2021

Role of Klotho in Hyperglycemia: Its Levels and Effects on Fibroblast Growth Factor Receptors, Glycolysis, and Glomerular Filtration.

Applications

Unspecified application

Species

Unspecified reactive species

Marlena Typiak,Tomasz Kulesza,Patrycja Rachubik,Dorota Rogacka,Irena Audzeyenka,Stefan Angielski,Moin A Saleem,Agnieszka Piwkowska

PloS one 16:e0245614 PubMed33481901

2021

A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Shreyas Jadhav,Sudipta Tripathi,Anil Chandrekar,Sushrut S Waikar,Li-Li Hsiao

BMC cardiovascular disorders 20:120 PubMed32138681

2020

Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Xiujuan Chen,Min Wu,Lin Li,Jiani Liu,Jing Shi,Tian Hong

Aging cell 18:e12913 PubMed30773786

2019

The angiotensin-(1-7)/Mas receptor axis protects from endothelial cell senescence via klotho and Nrf2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Romero,Álvaro San Hipólito-Luengo,Laura A Villalobos,Susana Vallejo,Inés Valencia,Patrycja Michalska,Natalia Pajuelo-Lozano,Isabel Sánchez-Pérez,Rafael León,José Luis Bartha,María Jesús Sanz,Jorge D Erusalimsky,Carlos F Sánchez-Ferrer,Tania Romacho,Concepción Peiró

Arthritis research & therapy 19:27 PubMed28183357

2017

Proangiogenic effects of soluble α-Klotho on systemic sclerosis dermal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Celestina Mazzotta,Mirko Manetti,Irene Rosa,Eloisa Romano,Jelena Blagojevic,Silvia Bellando-Randone,Cosimo Bruni,Gemma Lepri,Serena Guiducci,Lidia Ibba-Manneschi,Marco Matucci-Cerinic
View all publications
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