Recombinant Human KLRG1 protein (denatured) is a Human Fragment protein, in the 60 to 189 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S L C Q G S N Y S T C A S C P S C P D R W M K Y G N H C Y Y F S V E E K D W N S S L E F C L A R D S H L L V I T D N Q E M S L L Q V F L S E A F C W I G L R N N S G W R W E D G S P L N F S R I S S N S F V Q T C G A I N K N G L Q A S S C E V P L H W V C K K V R L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
Alternative names
CLEC15A, MAFA, MAFAL, KLRG1, Killer cell lectin-like receptor subfamily G member 1, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, Mast cell function-associated antigen
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Recombinant Human KLRG1 protein (denatured) is a Human Fragment protein, in the 60 to 189 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96E93-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S L C Q G S N Y S T C A S C P S C P D R W M K Y G N H C Y Y F S V E E K D W N S S L E F C L A R D S H L L V I T D N Q E M S L L Q V F L S E A F C W I G L R N N S G W R W E D G S P L N F S R I S S N S F V Q T C G A I N K N G L Q A S S C E V P L H W V C K K V R L
- Accession
- Q96E93
- Protein length
- Fragment
- Predicted molecular weight
- 17 kDa
- Amino acids
- 60 to 189
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLRG1 also known as killer cell lectin-like receptor G1 is a protein with a mass of about 30 kDa. It functions mechanically as an inhibitory receptor on natural killer (NK) cells and subsets of T cells. KLRG1 binds to classical cadherins like E-cadherin and N-cadherin modulating immune cell activity. Expression of KLRG1 is found mainly on mature NK cells and activated T cells particularly on those with an effector memory phenotype. Detecting KLRG1 expression often involves flow cytometry techniques using specific anti-KLRG1 antibodies.
- Biological function summary
KLRG1 serves as a modulator of immune responses by delivering inhibitory signals that regulate immune cell cytotoxicity and cytokine production. It is not part of a larger protein complex but interacts with other molecules on the cell surface to perform its role. The engagement of its ligands mediates the reduction of immune responses which is important during chronic infections and cancer to prevent excessive immune activation.
- Pathways
Several immune regulatory pathways involve KLRG1. It notably participates in the balance between immune activation and inhibition within the immune system. KLRG1 interacts with signaling pathways such as those regulated by T-cell receptor (TCR) signaling and NK cell activation. Related proteins in these pathways include CD226 and NKG2A which also play a role in determining the immune cell response.
- Associated diseases and disorders
KLRG1 has relevance in chronic infections and cancer due to its role in regulating immune cell activity. In chronic viral infections such as HIV and hepatitis KLRG1 expression is upregulated coinciding with T cell exhaustion. This marker is also significant in cancer where it contributes to the immune evasion mechanisms of tumors. The association with other proteins like PD-1 and TIM-3 further highlights KLRG1's function in these contexts serving as potential targets for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Human KLRG1 protein (denatured) (ab139253)
15% SDS-PAGE analysis of 3 µg ab139253.
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