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AB196419

Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus)

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Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1849 to 2094 aa range, expressed in Escherichia coli, with >76%, suitable for SDS-PAGE, FuncS.

View Alternative Names

ARA267, KMT3B, NSD1, Androgen receptor coactivator 267 kDa protein, Androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, Lysine N-methyltransferase 3B, Nuclear receptor-binding SET domain-containing protein 1, NR-binding SET domain-containing protein

2 Images
Functional Studies - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)
  • FuncS

Supplier Data

Functional Studies - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

Activity assay of ab196419. Assay was performed using a NSD1 Chemiluminescent Assay Kit with 400 μM S-adenosyl- methionine on biotinylated H3 peptide coated plates overnight at room temperature.

SDS-PAGE - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

SDS-PAGE of 3μg ab196419 (lane 1). Protein marker (lane 2).
4-20% gradient gel, coomassie staining.

Key facts

Purity

>76% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

Q96L73

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.12% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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Complementary Products

Primary Antibodies

AB222145

Anti-KMT3B / NSD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KMT3B / NSD1 antibody (AB222145)

  • 0
  • 0
Proteins & Peptides

AB198120

Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus)

Functional Studies - Recombinant human WHSC1/NSD2 protein (Catalytic domain) (GST tag N-Terminus) (AB198120)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75359

Anti-WHSC1/NSD2 antibody [29D1]

Trial Size

Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KAQKELRQLQEDRKNDKKPPPYKHIKVNRPIGRVQIFTADLSEIPRCNCKATDENPCGIDSECINRMLLYECHPTVCPAGGRCQNQCFSKRQYPEVEIFRTLQRGWGLRTKTDIKKGEFVNEYVGELIDEEECRARIRYAQEHDITNFYMLTLDKDRIIDAGPKGNYARFMNHCCQPNCETQKWSVNGDTRVGLFALSDIKAGTELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPIATEE","proteinLength":"Fragment","predictedMolecularWeight":"54 kDa","actualMolecularWeight":null,"aminoAcidEnd":2094,"aminoAcidStart":1849,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96L73","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT3B also known as NSD1 is a histone methyltransferase enzyme with a significant role in chromatin modification. This protein adds methyl groups to histone H3 at lysine 36 (H3K36). With a molecular mass around 280 kDa NSD1 is mostly expressed in tissues such as the brain and skeletal muscle. It plays an integral role in controlling gene expression and maintaining genomic stability through its actions on chromatin.
Biological function summary

NSD1 modulates transcription regulation by interacting with other nuclear proteins. As a part of the epigenetic regulation mechanism it is involved in complex processes of genome organization. By methylating H3K36 NSD1 affects chromatin dynamics and influences transcriptional activation and repression. NSD1 supports the orchestration of transcriptional changes in response to cellular signals and environmental stimuli.

Pathways

NSD1 interfaces with transcriptional regulation and chromatin remodeling pathways. Its enzymatic action on H3K36 links NSD1 to pathways governing cell growth and differentiation. Through its interactions NSD1 associates with proteins like MLL and SETD2 highlighting its role in the regulation of gene expression and cellular developmental pathways.

NSD1's dysfunction associates with Sotos syndrome and acute myeloid leukemia. Mutations in NSD1 lead to overgrowth and developmental delay characteristics observed in Sotos syndrome. In leukemia disrupted methylation patterns arise from NSD1 alterations affecting proteins like NUP98 contributing to oncogenic transformation and malignancy progression.
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Specifications

Form

Liquid

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General info

Function

Histone methyltransferase that dimethylates Lys-36 of histone H3 (H3K36me2). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase that dimethylates Lys-36 of histone H3 (H3K36me2). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context.
See full target information NSD1
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Product promise

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