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AB323152

Recombinant Human KMT4/Dot1L Protein Standard (His tag)

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Recombinant Human KMT4/Dot1L Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.

View Alternative Names

KIAA1814, KMT4, DOT1L, DOT1-like protein, Disruptor of telomeric silencing 1-like histone lysine methyltransferase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase

2 Images
Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

SDS-PAGE analysis of ab323152 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q8TEK3

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"49.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TEK3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).
See full target information DOT1L
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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