Recombinant Human KMT5C/SUV4-20h2 protein is a Human Fragment protein, in the 2 to 280 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:24396869, PubMed:28114273). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (PubMed:24396869). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Alternative names
SUV420H2, PP7130, KMT5C, Histone-lysine N-methyltransferase KMT5C, Lysine N-methyltransferase 5C, Lysine-specific methyltransferase 5C, Suppressor of variegation 4-20 homolog 2, [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B, [histone H4]-lysine20 N-methyltransferase KMT5B, Su(var)4-20 homolog 2, Suv4-20h2
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Recombinant Human KMT5C/SUV4-20h2 protein is a Human Fragment protein, in the 2 to 280 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q86Y97-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- Accession
- Q86Y97
- Protein length
- Fragment
- Amino acids
- 2 to 280
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:24396869, PubMed:28114273). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (PubMed:24396869). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
- Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT5C also known as SUV4-20h2 is a histone lysine methyltransferase. Mechanically it catalyzes the trimethylation of lysine 20 on histone H4 (H4K20me3) an important histone modification for DNA repair and regulation of chromatin structure. The molecular mass of KMT5C is approximately 48 kDa. KMT5C is expressed in various tissues with higher levels observed in the tissues involved in cell proliferation and differentiation like testes and bone marrow.
- Biological function summary
KMT5C influences chromatin condensation and thereby contributes to genomic stability. The protein often functions as part of larger complexes involved in epigenetic regulation working closely with other chromatin-modifying proteins. By methylating H4K20 KMT5C plays a significant role in regulating cell cycle progression notably during the DNA damage response and repair processes. This function assists in maintaining proper cellular function and preventing aberrant cellular proliferation.
- Pathways
KMT5C has significant involvement in the DNA damage repair pathway and the cell cycle regulation. Within these pathways it associates with proteins like 53BP1 an important player in DNA damage recognition and repair. KMT5C activity in histone modification integrates into the broader framework of chromatin remodeling necessary for efficient DNA repair and cell cycle control ensuring cellular maintenance in response to genomic insults.
- Associated diseases and disorders
Changes in KMT5C expression or function have associations with cancer and neurodevelopmental disorders. Aberrant methylation patterns caused by altered KMT5C activity can lead to genomic instability contributing to oncogenesis. Furthermore disturbances in its function may impact neural development potentially linking it to conditions such as autism spectrum disorder. KMT5C's relationship with proteins like E2F1 a regulator of cell cycle events highlights its relevance to these disease processes.
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1 product image
SDS-PAGE - Recombinant Human KMT5C/SUV4-20h2 protein (ab80290)
10% SDS-PAGE showing ab80290 at approximately 58kDa (5μg).
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