JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB123205

Recombinant Human KPNA2 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human KPNA2 protein is a Human Full Length protein, in the 1 to 529 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha

1 Images
SDS-PAGE - Recombinant Human KPNA2 protein (AB123205)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KPNA2 protein (AB123205)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P52292

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKVVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAENLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF","proteinLength":"Full Length","predictedMolecularWeight":"60.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":529,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P52292","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.
Biological function summary

KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.

Pathways

KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.

KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.
style
left-column

Specifications

Form

Liquid

Additional notes

ab123205 is purified by using conventional chromatography.

style
left-column

General info

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).

Sequence similarities

Belongs to the importin alpha family.

Post-translational modifications

Ubiquitinated. Deubiquitinated by USP22; leading to stabilization and increased activity.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).
See full target information KPNA2
style
left-column
style
left-column
style
right-column

Complementary Products

Biochemicals

AB120689

Dyngo® 4a highly potent dynamin inhibitor

Chemical Structure - Dyngo® 4a highly potent dynamin inhibitor (AB120689)

  • 0
  • 0
Primary Antibodies

AB167171

Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB167171)

  • 5
  • 2
Primary Antibodies

AB289858

Anti-KPNA2 antibody [EPR25248-95]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody [EPR25248-95] (AB289858)

  • 0
  • 0
Primary Antibodies

AB217966

Anti-PGE2 receptor EP4 subtype antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com