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AB116172

Recombinant Human KPNB1 protein

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Recombinant Human KPNB1 protein is a Human Full Length protein, in the 1 to 876 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

NTF97, KPNB1, Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97

1 Images
SDS-PAGE - Recombinant Human KPNB1 protein (AB116172)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KPNB1 protein (AB116172)

15% SDS-PAGE showing ab116172 at approximately 99.6kDa (3µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q14974

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as NTF97/Importin beta.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA","proteinLength":"Full Length","predictedMolecularWeight":"99.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":876,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14974","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNB1 also known as importin beta plays hard role in nucleocytoplasmic transport. This large protein with a mass approximately 97 kDa shuttles proteins and ribonucleoproteins into the nucleus. KPNB1 expression occurs widely in many tissues reflecting its essential function in cell operations. As a solenoid protein KPNB1 structure allows flexibility needed for diverse interactions with cargo proteins and nuclear pore complexes.
Biological function summary

Importin beta facilitates the import of nuclear localization signal (NLS)-bearing proteins into the nucleus. KPNB1 forms a complex with importin alpha to mediate import processes. Importin alpha binds to the NLS-containing cargo while KPNB1 interacts with the nuclear pore complex. This system oversees important regulation of several cellular processes including signal transduction and cell cycle progression. The protein's role helps to organize cellular response to various stimuli maintaining cellular homeostasis.

Pathways

KPNB1 contributes to regulation in the nuclear import and export pathways. It tightly associates with Ran-GTPase a protein involved in the Ran pathway. This interaction supports directionality and specificity of energy-dependent nuclear transport. Additionally KPNB1 associates with proteins like NUP50 and NUP98 functioning as a gatekeeper at the nuclear pore complex by controlling cargo passage. Through these pathways KPNB1 balances cellular imports and exports critical for cell health and functionality.

KPNB1 has links to cancer notably in tumorigenesis and cancer progression. Studies show aberrant KPNB1 expression affects the nuclear import machinery supporting cancer cell proliferation and survival. KPNB1 interacts with oncogenic proteins such as c-Myc influencing transcription regulation in tumor cells. Furthermore KPNB1 holds relevance in neurodegenerative diseases like Alzheimer's where impaired transport function contributes to pathological accumulation of proteins in neuronal cells.
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Specifications

Form

Liquid

Additional notes

ab116172 is purified using conventional chromatography techniques.

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General info

Function

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Acting autonomously, serves itself as NLS receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607, PubMed : 9687515). In association with IPO7, mediates the nuclear import of H1 histone (PubMed : 10228156). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). Imports MRTFA, SNAI1 and PRKCI into the nucleus (PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649).. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Sequence similarities

Belongs to the importin beta family. Importin beta-1 subfamily.

Post-translational modifications

Mono-ADP-ribosylated by PARP16.

Subcellular localisation

Nucleus envelope

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Product protocols

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Target data

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Acting autonomously, serves itself as NLS receptor (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 10228156, PubMed : 11682607, PubMed : 11891849, PubMed : 19386897, PubMed : 24699649, PubMed : 7615630, PubMed : 9687515). Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607, PubMed : 9687515). In association with IPO7, mediates the nuclear import of H1 histone (PubMed : 10228156). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). Imports MRTFA, SNAI1 and PRKCI into the nucleus (PubMed : 11891849, PubMed : 19386897, PubMed : 20818336, PubMed : 24699649).. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
See full target information KPNB1
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