Recombinant Human KPNB1 protein is a Human Full Length protein, in the 1 to 876 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E L I T I L E K T V S P D R L E L E A A Q K F L E R A A V E N L P T F L V E L S R V L A N P G N S Q V A R V A A G L Q I K N S L T S K D P D I K A Q Y Q Q R W L A I D A N A R R E V K N Y V L Q T L G T E T Y R P S S A S Q C V A G I A C A E I P V N Q W P E L I P Q L V A N V T N P N S T E H M K E S T L E A I G Y I C Q D I D P E Q L Q D K S N E I L T A I I Q G M R K E E P S N N V K L A A T N A L L N S L E F T K A N F D K E S E R H F I M Q V V C E A T Q C P D T R V R V A A L Q N L V K I M S L Y Y Q Y M E T Y M G P A L F A I T I E A M K S D I D E V A L Q G I E F W S N V C D E E M D L A I E A S E A A E Q G R P P E H T S K F Y A K G A L Q Y L V P I L T Q T L T K Q D E N D D D D D W N P C K A A G V C L M L L A T C C E D D I V P H V L P F I K E H I K N P D W R Y R D A A V M A F G C I L E G P E P S Q L K P L V I Q A M P T L I E L M K D P S V V V R D T A A W T V G R I C E L L P E A A I N D V Y L A P L L Q C L I E G L S A E P R V A S N V C W A F S S L A E A A Y E A A D V A D D Q E E P A T Y C L S S S F E L I V Q K L L E T T D R P D G H Q N N L R S S A Y E S L M E I V K N S A K D C Y P A V Q K T T L V I M E R L Q Q V L Q M E S H I Q S T S D R I Q F N D L Q S L L C A T L Q N V L R K V Q H Q D A L Q I S D V V M A S L L R M F Q S T A G S G G V Q E D A L M A V S T L V E V L G G E F L K Y M E A F K P F L G I G L K N Y A E Y Q V C L A A V G L V G D L C R A L Q S N I I P F C D E V M Q L L L E N L G N E N V H R S V K P Q I L S V F G D I A L A I G G E F K K Y L E V V L N T L Q Q A S Q A Q V D K S D Y D M V D Y L N E L R E S C L E A Y T G I V Q G L K G D Q E N V H P D V M L V Q P R V E F I L S F I D H I A G D E D H T D G V V A C A A G L I G D L C T A F G K D V L K L V E A R P M I H E L L T E G R R S K T N K A K T L A T W A T K E L R K L K N Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.
Alternative names
Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97, KPNB1, NTF97
Recommended products
Recombinant Human KPNB1 protein is a Human Full Length protein, in the 1 to 876 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q14974-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M E L I T I L E K T V S P D R L E L E A A Q K F L E R A A V E N L P T F L V E L S R V L A N P G N S Q V A R V A A G L Q I K N S L T S K D P D I K A Q Y Q Q R W L A I D A N A R R E V K N Y V L Q T L G T E T Y R P S S A S Q C V A G I A C A E I P V N Q W P E L I P Q L V A N V T N P N S T E H M K E S T L E A I G Y I C Q D I D P E Q L Q D K S N E I L T A I I Q G M R K E E P S N N V K L A A T N A L L N S L E F T K A N F D K E S E R H F I M Q V V C E A T Q C P D T R V R V A A L Q N L V K I M S L Y Y Q Y M E T Y M G P A L F A I T I E A M K S D I D E V A L Q G I E F W S N V C D E E M D L A I E A S E A A E Q G R P P E H T S K F Y A K G A L Q Y L V P I L T Q T L T K Q D E N D D D D D W N P C K A A G V C L M L L A T C C E D D I V P H V L P F I K E H I K N P D W R Y R D A A V M A F G C I L E G P E P S Q L K P L V I Q A M P T L I E L M K D P S V V V R D T A A W T V G R I C E L L P E A A I N D V Y L A P L L Q C L I E G L S A E P R V A S N V C W A F S S L A E A A Y E A A D V A D D Q E E P A T Y C L S S S F E L I V Q K L L E T T D R P D G H Q N N L R S S A Y E S L M E I V K N S A K D C Y P A V Q K T T L V I M E R L Q Q V L Q M E S H I Q S T S D R I Q F N D L Q S L L C A T L Q N V L R K V Q H Q D A L Q I S D V V M A S L L R M F Q S T A G S G G V Q E D A L M A V S T L V E V L G G E F L K Y M E A F K P F L G I G L K N Y A E Y Q V C L A A V G L V G D L C R A L Q S N I I P F C D E V M Q L L L E N L G N E N V H R S V K P Q I L S V F G D I A L A I G G E F K K Y L E V V L N T L Q Q A S Q A Q V D K S D Y D M V D Y L N E L R E S C L E A Y T G I V Q G L K G D Q E N V H P D V M L V Q P R V E F I L S F I D H I A G D E D H T D G V V A C A A G L I G D L C T A F G K D V L K L V E A R P M I H E L L T E G R R S K T N K A K T L A T W A T K E L R K L K N Q A
- Accession
- Protein length
Full Length
- Predicted molecular weight
99.6 kDa
- Amino acids
1 to 876
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab116172 is purified using conventional chromatography techniques.
General info
- Function
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.
- Sequence similarities
Belongs to the importin beta family. Importin beta-1 subfamily.
- Post-translational modifications
Mono-ADP-ribosylated by PARP16.
- Subcellular localisation
Nucleus envelope
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Previously labelled as NTF97/Importin beta.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KPNB1 also known as importin beta plays hard role in nucleocytoplasmic transport. This large protein with a mass approximately 97 kDa shuttles proteins and ribonucleoproteins into the nucleus. KPNB1 expression occurs widely in many tissues reflecting its essential function in cell operations. As a solenoid protein KPNB1 structure allows flexibility needed for diverse interactions with cargo proteins and nuclear pore complexes.
- Biological function summary
Importin beta facilitates the import of nuclear localization signal (NLS)-bearing proteins into the nucleus. KPNB1 forms a complex with importin alpha to mediate import processes. Importin alpha binds to the NLS-containing cargo while KPNB1 interacts with the nuclear pore complex. This system oversees important regulation of several cellular processes including signal transduction and cell cycle progression. The protein's role helps to organize cellular response to various stimuli maintaining cellular homeostasis.
- Pathways
KPNB1 contributes to regulation in the nuclear import and export pathways. It tightly associates with Ran-GTPase a protein involved in the Ran pathway. This interaction supports directionality and specificity of energy-dependent nuclear transport. Additionally KPNB1 associates with proteins like NUP50 and NUP98 functioning as a gatekeeper at the nuclear pore complex by controlling cargo passage. Through these pathways KPNB1 balances cellular imports and exports critical for cell health and functionality.
- Associated diseases and disorders
KPNB1 has links to cancer notably in tumorigenesis and cancer progression. Studies show aberrant KPNB1 expression affects the nuclear import machinery supporting cancer cell proliferation and survival. KPNB1 interacts with oncogenic proteins such as c-Myc influencing transcription regulation in tumor cells. Furthermore KPNB1 holds relevance in neurodegenerative diseases like Alzheimer's where impaired transport function contributes to pathological accumulation of proteins in neuronal cells.
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1 product image
SDS-PAGE - Recombinant Human KPNB1 protein (ab116172)
15% SDS-PAGE showing ab116172 at approximately 99.6kDa (3µg).
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