Recombinant Human KREMEN1 protein (His tag) is a Human Fragment protein, in the 1 to 394 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A P P A A R L A L L S A A A L T L A A R P A P S P G L G P G P E C F T A N G A D Y R G T Q N W T A L Q G G K P C L F W N E T F Q H P Y N T L K Y P N G E G G L G E H N Y C R N P D G D V S P W C Y V A E H E D G V Y W K Y C E I P A C Q M P G N L G C Y K D H G N P P P L T G T S K T S N K L T I Q T C I S F C R S Q R F K F A G M E S G Y A C F C G N N P D Y W K Y G E A A S T E C N S V C F G D H T Q P C G G D G R I I L F D T L V G A C G G N Y S A M S S V V Y S P D F P D T Y A T G R V C Y W T I R V P G A S H I H F S F P L F D I R D S A D M V E L L D G Y T H R V L A R F H G R S R P P L S F N V S L D F V I L Y F F S D R I N Q A Q G F A V L Y Q A V K E E L P Q E R P A V N Q T V A E V I T E Q A N L S V S A A R S S K V L Y V I T T S P S H P P Q T V P G S N S W A P P M G A G S H R V E G W T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.
Alternative names
KREMEN, KRM1, KREMEN1, Kremen protein 1, Dickkopf receptor, Kringle domain-containing transmembrane protein 1, Kringle-containing protein marking the eye and the nose
Recommended products
Recombinant Human KREMEN1 protein (His tag) is a Human Fragment protein, in the 1 to 394 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96MU8-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A P P A A R L A L L S A A A L T L A A R P A P S P G L G P G P E C F T A N G A D Y R G T Q N W T A L Q G G K P C L F W N E T F Q H P Y N T L K Y P N G E G G L G E H N Y C R N P D G D V S P W C Y V A E H E D G V Y W K Y C E I P A C Q M P G N L G C Y K D H G N P P P L T G T S K T S N K L T I Q T C I S F C R S Q R F K F A G M E S G Y A C F C G N N P D Y W K Y G E A A S T E C N S V C F G D H T Q P C G G D G R I I L F D T L V G A C G G N Y S A M S S V V Y S P D F P D T Y A T G R V C Y W T I R V P G A S H I H F S F P L F D I R D S A D M V E L L D G Y T H R V L A R F H G R S R P P L S F N V S L D F V I L Y F F S D R I N Q A Q G F A V L Y Q A V K E E L P Q E R P A V N Q T V A E V I T E Q A N L S V S A A R S S K V L Y V I T T S P S H P P Q T V P G S N S W A P P M G A G S H R V E G W T
- Accession
- Q96MU8
- Protein length
- Fragment
- Predicted molecular weight
- 42.6 kDa
- Amino acids
- 1 to 394
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KREMEN1 also known as Kringle Containing Transmembrane Protein 1 is a transmembrane protein with a mass of approximately 56 kDa. It acts as a receptor involved in the regulation of the Wnt-signaling pathway. This protein participates in modulating cellular functions and development. KREMEN1 expression occurs in various tissues with significant presence noted in the brain kidney and lung. Its role as an antagonist in the pathway marks its importance in maintaining cellular homeostasis.
- Biological function summary
KREMEN1 interacts critically with other components of the Wnt pathway such as Dickkopf-1 (DKK1) to mediate cellular signaling. These interactions form a receptor complex with Lipoprotein receptor-related protein 6 (LRP6) which plays a significant role in inhibiting Wnt/beta-catenin signaling. This inhibition is important for processes such as cell differentiation and proliferation. KREMEN1's involvement in these processes highlights its contribution to controlling cellular behavior.
- Pathways
KREMEN1 participates primarily in the Wnt signaling pathway. It regulates this pathway by affecting the LRP6 co-receptor in conjunction with DKK1. Another significant pathway involving KREMEN1 is the bone morphogenetic protein (BMP) signaling pathway. Here its interactions illustrate a complex interplay with proteins like noggin and chordin contributing to embryo development and bone formation processes.
- Associated diseases and disorders
Alterations in KREMEN1 expression or function can relate to cancer and bone disorders. In cancers aberrant Wnt signaling often facilitated through dysregulation of KREMEN1 and DKK1 interaction contributes to tumor development and progression. Bone developmental disorders such as osteoporosis may arise from disruptions in KREMEN1's role in bone morphogenetic protein pathways. Understanding KREMEN1's connection with these conditions proves important in therapeutic development and research.
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SDS-PAGE - Recombinant Human KREMEN1 protein (His tag) (ab276720)
SDS-PAGE analysis of ab276720
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