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AB268720

Recombinant Human KSR2 protein (Tagged)

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Recombinant Human KSR2 protein (Tagged) is a Human Fragment protein, in the 554 to 950 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE.

View Alternative Names

Kinase suppressor of Ras 2, hKSR2, KSR2

1 Images
SDS-PAGE - Recombinant Human KSR2 protein (Tagged) (AB268720)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KSR2 protein (Tagged) (AB268720)

SDS-PAGE analysis of ab268720.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q6VAB6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":950,"aminoAcidStart":554,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q6VAB6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KSR2 protein also known as Kinase Suppressor of Ras 2 operates as a scaffold protein. It functions by assembling a protein complex that is important for signal transduction. KSR2 has a molecular weight of approximately 104 kDa. This protein is widely expressed in various tissues most notably in the brain and adipose tissue where it plays key roles in cellular communication and metabolic regulation.
Biological function summary

Kinase Suppressor of Ras 2 participates in the scaffolding of protein complexes required for MEK/ERK signaling. KSR2 contributes to the regulation of cellular energy homeostasis and metabolic processes. It also acts as a facilitator that enhances the signaling efficiency of the MAPK pathway allowing proper transduction of growth and developmental signals. Its role in the modulation of energy balance involves effects on both glucose and fatty acid metabolism.

Pathways

KSR2 interacts notably with the MEK/ERK and AMPK signaling pathways. KSR2 modulates the MAPK pathway facilitating the phosphorylation of ERK an essential step for transmitting signals from membrane receptors to the nucleus. In the AMPK pathway KSR2 interacts indirectly with AMPK-related proteins impacting energy balance and cellular stress responses. Through these pathways KSR2 links with key proteins like Raf MEK and LKB1 important for signal transduction and energy regulation.

KSR2 plays a significant role in obesity and type 2 diabetes. Mutations in KSR2 can lead to severe obesity accompanied by insulin resistance indicating its importance in regulating metabolism and energy consumption. Through these disorders KSR2 links with proteins such as AMPK which influences energy homeostasis and insulin signaling. Addressing KSR2 dysfunction may have therapeutic potential for metabolic diseases highlighting its importance in health and disease.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed : 29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylated on Ser-474 by MARK3.

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Product protocols

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Target data

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed : 29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
See full target information Kinase suppressor of Ras 2
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