Recombinant Human kynurenine 3-monooxygenase protein is a Human Full Length protein, in the 1 to 407 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M D S S V I Q R K K V A V I G G G L V G S L Q A C F L A K R N F Q I D V Y E A R E D T R V A T F T R G R S I N L A L S H R G R Q A L K A V G L E D Q I V S Q G I P M R A R M I H S L S G K K S A I P Y G T K S Q Y I L S V S R E N L N K D L L T A A E K Y P N V K M H F N H R L L K C N P E E G M I T V L G S D K V P K D V T C D L I V G C D G A Y S T V R S H L M K K P R F D Y S Q Q Y I P H G Y M E L T I P P K N G D Y A M E P N Y L H I W P R N T F M M I A L P N M N K S F T C T L F M P F E E F E K L L T S N D V V D F F Q K Y F P D A I P L I G E K L L V Q D F F L L P A Q P M I S V K C S S F H F K S H C V L L G D A A H A I V P F F G Q G M N A G F E D C L V F D E L M D K F S N D L S L C L P V F S R L R I P D D H A I S D L S M Y N Y I E K N M E R F L H A I M P S T F I P L Y T M V T F S R I R Y H E A V Q R W H W Q K R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed:23575632, PubMed:26752518, PubMed:28604669, PubMed:29208702, PubMed:29429898). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).
Alternative names
Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, KMO
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Recombinant Human kynurenine 3-monooxygenase protein is a Human Full Length protein, in the 1 to 407 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O15229-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D S S V I Q R K K V A V I G G G L V G S L Q A C F L A K R N F Q I D V Y E A R E D T R V A T F T R G R S I N L A L S H R G R Q A L K A V G L E D Q I V S Q G I P M R A R M I H S L S G K K S A I P Y G T K S Q Y I L S V S R E N L N K D L L T A A E K Y P N V K M H F N H R L L K C N P E E G M I T V L G S D K V P K D V T C D L I V G C D G A Y S T V R S H L M K K P R F D Y S Q Q Y I P H G Y M E L T I P P K N G D Y A M E P N Y L H I W P R N T F M M I A L P N M N K S F T C T L F M P F E E F E K L L T S N D V V D F F Q K Y F P D A I P L I G E K L L V Q D F F L L P A Q P M I S V K C S S F H F K S H C V L L G D A A H A I V P F F G Q G M N A G F E D C L V F D E L M D K F S N D L S L C L P V F S R L R I P D D H A I S D L S M Y N Y I E K N M E R F L H A I M P S T F I P L Y T M V T F S R I R Y H E A V Q R W H W Q K R
- Accession
- O15229
- Protein length
- Full Length
- Predicted molecular weight
- 70.51 kDa
- Amino acids
- 1 to 407
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed:23575632, PubMed:26752518, PubMed:28604669, PubMed:29208702, PubMed:29429898). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).
- Sequence similarities
Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
- Subcellular localisation
- Mitochondrion outer membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kynurenine 3-monooxygenase (KMO) also known as kynurenine 3-hydroxylase is an enzyme that plays a mechanical role in the kynurenine pathway. This enzyme catalyzes the conversion of L-kynurenine to 3-hydroxykynurenine. KMO has a molecular mass of approximately 55 kDa. It is primarily expressed in the liver and kidney tissues but can also be found in other organs at lower levels. The structure of KMO contains a flavin adenine dinucleotide (FAD) binding domain which is essential for its oxidoreductase activity.
- Biological function summary
KMO participates in the degradation of tryptophan significantly impacting the biosynthesis of nicotinamide adenine dinucleotide (NAD+). KMO is not known to be part of any protein complexes but its enzymatic activity is important for the production of downstream metabolites that influence various physiological processes. This activity affects immune response neurogenesis and energy metabolism highlighting its role in maintaining metabolic balance.
- Pathways
KMO is an integral component of the kynurenine pathway which is critical for tryptophan catabolism. It associates closely with other enzymes like kynureninase and kynurenine aminotransferase. KMO exerts its function by regulating the levels of neuroactive and immunoactive metabolites in the brain and peripheral tissues. Through its role in the kynurenine pathway KMO influences cellular signaling and energy production connecting to broader metabolic pathways.
- Associated diseases and disorders
KMO plays a significant role in neurodegenerative diseases such as Alzheimer's and Huntington's disease. Altered KMO activity leads to an imbalance of kynurenine pathway metabolites impacting neuronal health. The enzyme's dysfunction also associates with psychiatric disorders like schizophrenia. The relationship between KMO and other kynurenine pathway enzymes like kynurenine aminotransferase suggests that modulating its activity could present therapeutic opportunities for these conditions.
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1 product image
SDS-PAGE - Recombinant Human kynurenine 3-monooxygenase protein (ab152891)
12.5% SDS-PAGE analysis of ab152891 stained with Coomassie Blue.
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