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AB271578

Recombinant human kynurenine 3-monooxygenase protein (Active)

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Recombinant human kynurenine 3-monooxygenase protein (Active) is a Human Full Length protein, in the 1 to 486 aa range, expressed in Baculovirus infected Sf9 cells, with >60%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, KMO

2 Images
Functional Studies - Recombinant human kynurenine 3-monooxygenase protein (Active) (AB271578)
  • FuncS

Supplier Data

Functional Studies - Recombinant human kynurenine 3-monooxygenase protein (Active) (AB271578)

Specific activity of ab271578 was 100 pmol/min/μg.

SDS-PAGE - Recombinant human kynurenine 3-monooxygenase protein (Active) (AB271578)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human kynurenine 3-monooxygenase protein (Active) (AB271578)

SDS-PAGE analysis of ab271578.

Key facts

Purity

>60% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His-DDDDK tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab271578 was 100 pmol/min/μg.

ab271578 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 μM NADPH, 400 μM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm in a plate reader.

Accession

O15229

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.01% TCEP HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDSSVIQRKKVAVIGGGLVGSLQACFLAKRNFQIDVYEAREDTRVATFTRGRSINLALSHRGRQALKAVGLEDQIVSQGIPMRARMIHSLSGKKSAIPYGTKSQYILSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCNPEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEPNYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLLPAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRIPDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTYLLIHYMSPRSFLRLRRPWNWIAHFRNTTCFPAKAVDSLEQISNLISR","proteinLength":"Full Length","predictedMolecularWeight":"58 kDa","actualMolecularWeight":null,"aminoAcidEnd":486,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O15229","tags":[{"tag":"His-DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kynurenine 3-monooxygenase (KMO) also known as kynurenine 3-hydroxylase is an enzyme that plays a mechanical role in the kynurenine pathway. This enzyme catalyzes the conversion of L-kynurenine to 3-hydroxykynurenine. KMO has a molecular mass of approximately 55 kDa. It is primarily expressed in the liver and kidney tissues but can also be found in other organs at lower levels. The structure of KMO contains a flavin adenine dinucleotide (FAD) binding domain which is essential for its oxidoreductase activity.
Biological function summary

KMO participates in the degradation of tryptophan significantly impacting the biosynthesis of nicotinamide adenine dinucleotide (NAD+). KMO is not known to be part of any protein complexes but its enzymatic activity is important for the production of downstream metabolites that influence various physiological processes. This activity affects immune response neurogenesis and energy metabolism highlighting its role in maintaining metabolic balance.

Pathways

KMO is an integral component of the kynurenine pathway which is critical for tryptophan catabolism. It associates closely with other enzymes like kynureninase and kynurenine aminotransferase. KMO exerts its function by regulating the levels of neuroactive and immunoactive metabolites in the brain and peripheral tissues. Through its role in the kynurenine pathway KMO influences cellular signaling and energy production connecting to broader metabolic pathways.

KMO plays a significant role in neurodegenerative diseases such as Alzheimer's and Huntington's disease. Altered KMO activity leads to an imbalance of kynurenine pathway metabolites impacting neuronal health. The enzyme's dysfunction also associates with psychiatric disorders like schizophrenia. The relationship between KMO and other kynurenine pathway enzymes like kynurenine aminotransferase suggests that modulating its activity could present therapeutic opportunities for these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed : 23575632, PubMed : 26752518, PubMed : 28604669, PubMed : 29208702, PubMed : 29429898). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).

Sequence similarities

Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed : 23575632, PubMed : 26752518, PubMed : 28604669, PubMed : 29208702, PubMed : 29429898). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).
See full target information kynurenine 3-monooxygenase
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