JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB271581

Recombinant human L-Kynurenine Hydrolase protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human L-Kynurenine Hydrolase protein (Active) is a Human Full Length protein, in the 1 to 465 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Kynureninase, L-kynurenine hydrolase, KYNU

2 Images
Functional Studies - Recombinant human L-Kynurenine Hydrolase protein (Active) (AB271581)
  • FuncS

Supplier Data

Functional Studies - Recombinant human L-Kynurenine Hydrolase protein (Active) (AB271581)

Specific activity of ab271581.

IC50 = 5.5 μM.

SDS-PAGE - Recombinant human L-Kynurenine Hydrolase protein (Active) (AB271581)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human L-Kynurenine Hydrolase protein (Active) (AB271581)

SDS-PAGE analysis of ab271581.

Key facts

Purity

>85% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥75 pmol/min/μg.

Accession

Q16719

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.53% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.00005% PLP, 0.00003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"EPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPALVGWFGHELSTRFKMDNKLQLIPGVCGFRISNPPILLVCSLHASLEIFKQATMKALRKKSVLLTGYLEYLIKHNYGKDKAATKKPVVNIITPSHVEERGCQLTITFSVPNKDVFQELEKRGVVCDKRNPNGIRVAPVPLYNSFHDVYKFTNLLTSILDSAETKN","proteinLength":"Full Length","predictedMolecularWeight":"53 kDa","actualMolecularWeight":null,"aminoAcidEnd":465,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q16719","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L-Kynurenine Hydrolase also known as L-Kynureninase is an enzyme with a mass of approximately 47 kDa. It acts as an important player in the kynurenine pathway by catalyzing the hydrolysis of L-kynurenine into anthranilic acid. This enzymatic activity is important for the degradation of tryptophan. L-Kynureninase is expressed in various tissues including the liver and brain reflecting its importance in systemic metabolic processes.
Biological function summary

L-Kynurenine Hydrolase participates in tryptophan catabolism affecting amino acid metabolism. It operates within the larger enzyme complex involved in the kynurenine pathway which is important for producing metabolites that have neurological and immunological functions. The activity of L-Kynureninase can influence the levels of pathway intermediates which are related to neurotransmitter synthesis and immune responses.

Pathways

This enzyme significantly contributes to the kynurenine pathway which is a major route for tryptophan metabolism. The pathway provides a link to the biosynthesis of nicotinamide adenine dinucleotide (NAD+) an essential coenzyme in cellular respiration. Proteins such as kynurenine 3-monooxygenase and aminocarboxymuconate semialdehyde decarboxylase are closely related to L-Kynureninase in these pathways interacting at different points in the metabolic sequence.

Altered L-Kynurenine Hydrolase activity is associated with conditions like neurodegenerative diseases and certain autoimmune disorders. For instance disruptions in its function can contribute to the development of neurological disorders such as Alzheimer's disease due to imbalanced neuroactive metabolite levels. It also has a connection with proteins like quinolinic acid a neurotoxin that may accumulate when L-Kynureninase activity is impaired linking it to potential damage in neural tissues.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.

Sequence similarities

Belongs to the kynureninase family.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
See full target information Kynureninase
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB211098

Tryptophan Assay Kit (Fluorometric)

Functional Studies - Tryptophan Assay Kit (Fluorometric) (AB211098)

  • 0
  • 0
Proteins & Peptides

AB271578

Recombinant human kynurenine 3-monooxygenase protein (Active)

Functional Studies - Recombinant human kynurenine 3-monooxygenase protein (Active) (AB271578)

  • 0
  • 0
Proteins & Peptides

AB198661

Recombinant Human WTAP protein

  • 0
  • 0
Proteins & Peptides

AB271721

Recombinant human PRMT5 + MEP50 protein (Active)

Functional Studies - Recombinant human PRMT5 + MEP50 protein (Active) (AB271721)

  • 0
  • 0
Proteins & Peptides

AB163782

Recombinant Human VISTA protein

SDS-PAGE - Recombinant Human VISTA protein (AB163782)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com