Recombinant Human L1CAM protein (His tag) is a Human Fragment protein, in the 20 to 1115 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
I Q I P E E L M E P P V I T E Q S P R R L V V F P T D D I S L K C E A S G K P E V Q F R W T R D G V H F K P K E E L G V T V Y Q S P H S G S F T I T G N N S N F A Q R F Q G I Y R C F A S N K L G T A M S H E I R L M A E G A P K W P K E T V K P V E V E E G E S V V L P C N P P P S A E P L R I Y W M N S K I L H I K Q D E R V T M G Q N G N L Y F A N V L T S D N H S D Y I C H A H F P G T R T I I Q K E P I D L R V K A T N S M I D R K P R L L F P T N S S S H L V A L Q G Q P L V L E C I A E G F P T P T I K W L R P S G P M P A D R V T Y Q N H N K T L Q L L K V G E E D D G E Y R C L A E N S L G S A R H A Y Y V T V E A A P Y W L H K P Q S H L Y G P G E T A R L D C Q V Q G R P Q P E V T W R I N G I P V E E L A K D Q K Y R I Q R G A L I L S N V Q P S D T M V T Q C E A R N R H G L L L A N A Y I Y V V Q L P A K I L T A D N Q T Y M A V Q G S T A Y L L C K A F G A P V P S V Q W L D E D G T T V L Q D E R F F P Y A N G T L G I R D L Q A N D T G R Y F C L A A N D Q N N V T I M A N L K V K D A T Q I T Q G P R S T I E K K G S R V T F T C Q A S F D P S L Q P S I T W R G D G R D L Q E L G D S D K Y F I E D G R L V I H S L D Y S D Q G N Y S C V A S T E L D V V E S R A Q L L V V G S P G P V P R L V L S D L H L L T Q S Q V R V S W S P A E D H N A P I E K Y D I E F E D K E M A P E K W Y S L G K V P G N Q T S T T L K L S P Y V H Y T F R V T A I N K Y G P G E P S P V S E T V V T P E A A P E K N P V D V K G E G N E T T N M V I T W K P L R W M D W N A P Q V Q Y R V Q W R P Q G T R G P W Q E Q I V S D P F L V V S N T S T F V P Y E I K V Q A V N S Q G K G P E P Q V T I G Y S G E D Y P Q A I P E L E G I E I L N S S A V L V K W R P V D L A Q V K G H L R G Y N V T Y W R E G S Q R K H S K R H I H K D H V V V P A N T T S V I L S G L R P Y S S Y H L E V Q A F N G R G S G P A S E F T F S T P E G V P G H P E A L H L E C Q S N T S L L L R W Q P P L S H N G V L T G Y V L S Y H P L D E G G K G Q L S F N L R D P E L R T H N L T D L S P H L R Y R F Q L Q A T T K E G P G E A I V R E G G T M A L S G I S D F G N I S A T A G E N Y S V V S W V P K E G Q C N F R F H I L F K A L G E E K G G A S L S P Q Y V S Y N Q S S Y T Q W D L Q P D T D Y E I H L F K E R M F R H Q M A V K T N G T G R V R L P P A G F A T E L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Alternative names
CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1
Recommended products
Recombinant Human L1CAM protein (His tag) is a Human Fragment protein, in the 20 to 1115 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P32004-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- I Q I P E E L M E P P V I T E Q S P R R L V V F P T D D I S L K C E A S G K P E V Q F R W T R D G V H F K P K E E L G V T V Y Q S P H S G S F T I T G N N S N F A Q R F Q G I Y R C F A S N K L G T A M S H E I R L M A E G A P K W P K E T V K P V E V E E G E S V V L P C N P P P S A E P L R I Y W M N S K I L H I K Q D E R V T M G Q N G N L Y F A N V L T S D N H S D Y I C H A H F P G T R T I I Q K E P I D L R V K A T N S M I D R K P R L L F P T N S S S H L V A L Q G Q P L V L E C I A E G F P T P T I K W L R P S G P M P A D R V T Y Q N H N K T L Q L L K V G E E D D G E Y R C L A E N S L G S A R H A Y Y V T V E A A P Y W L H K P Q S H L Y G P G E T A R L D C Q V Q G R P Q P E V T W R I N G I P V E E L A K D Q K Y R I Q R G A L I L S N V Q P S D T M V T Q C E A R N R H G L L L A N A Y I Y V V Q L P A K I L T A D N Q T Y M A V Q G S T A Y L L C K A F G A P V P S V Q W L D E D G T T V L Q D E R F F P Y A N G T L G I R D L Q A N D T G R Y F C L A A N D Q N N V T I M A N L K V K D A T Q I T Q G P R S T I E K K G S R V T F T C Q A S F D P S L Q P S I T W R G D G R D L Q E L G D S D K Y F I E D G R L V I H S L D Y S D Q G N Y S C V A S T E L D V V E S R A Q L L V V G S P G P V P R L V L S D L H L L T Q S Q V R V S W S P A E D H N A P I E K Y D I E F E D K E M A P E K W Y S L G K V P G N Q T S T T L K L S P Y V H Y T F R V T A I N K Y G P G E P S P V S E T V V T P E A A P E K N P V D V K G E G N E T T N M V I T W K P L R W M D W N A P Q V Q Y R V Q W R P Q G T R G P W Q E Q I V S D P F L V V S N T S T F V P Y E I K V Q A V N S Q G K G P E P Q V T I G Y S G E D Y P Q A I P E L E G I E I L N S S A V L V K W R P V D L A Q V K G H L R G Y N V T Y W R E G S Q R K H S K R H I H K D H V V V P A N T T S V I L S G L R P Y S S Y H L E V Q A F N G R G S G P A S E F T F S T P E G V P G H P E A L H L E C Q S N T S L L L R W Q P P L S H N G V L T G Y V L S Y H P L D E G G K G Q L S F N L R D P E L R T H N L T D L S P H L R Y R F Q L Q A T T K E G P G E A I V R E G G T M A L S G I S D F G N I S A T A G E N Y S V V S W V P K E G Q C N F R F H I L F K A L G E E K G G A S L S P Q Y V S Y N Q S S Y T Q W D L Q P D T D Y E I H L F K E R M F R H Q M A V K T N G T G R V R L P P A G F A T E L E H H H H H H
- Accession
- P32004
- Protein length
- Fragment
- Predicted molecular weight
- 123.6 kDa
- Amino acids
- 20 to 1115
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
- Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
- Biological function summary
L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.
- Pathways
L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.
- Associated diseases and disorders
L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human L1CAM protein (His tag) (ab222980)
15% SDS-PAGE analysis of 3 μg ab222980.
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