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AB216180

Recombinant Human L3MBTL1 protein (His tag)

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Recombinant Human L3MBTL1 protein (His tag) is a Human Fragment protein, in the 191 to 530 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

KIAA0681, L3MBT, L3MBTL, L3MBTL1, Lethal(3)malignant brain tumor-like protein 1, H-l(3)mbt, H-l(3)mbt protein, L(3)mbt-like, L(3)mbt protein homolog

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SDS-PAGE - Recombinant Human L3MBTL1 protein (His tag) (AB216180)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human L3MBTL1 protein (His tag) (AB216180)

4-20% SDS-PAGE analysis of 2 μg ab216180

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y468

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"EWSSSQPATGEKKECWSWESYLEEQKAITAPVSLFQDSQAVTHNKNGFKLGMKLEGIDPQHPSMYFILTVAEVCGYRLRLHFDGYSECHDFWVNANSPDIHPAGWFEKTGHKLQPPKGYKEEEFSWSQYLRSTRAQAAPKHLFVSQSHSPPPLGFQVGMKLEAVDRMNPSLVCVASVTDVVDSRFLVHFDNWDDTYDYWCDPSSPYIHPVGWCQKQGKPLTPPQDYPDPDNFCWEKYLEETGASAVPTWAFKVRPPHSFLVNMKLEAVDRRNPALIRVASVEDVEDHRIKIHFDGWSHGYDFWIDADHPDIHPAGWCSKTGHPLQPPLGPREPSSASPGG","proteinLength":"Fragment","predictedMolecularWeight":"39.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":530,"aminoAcidStart":191,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y468","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L3MBTL1 also known as the MBT domain-containing protein L3MBTL1 is a protein that plays a role in chromatin remodeling. This protein has a molecular mass of approximately 83 kDa. It is expressed in various tissues including hematopoietic progenitor cells and the brain. L3MBTL1 interacts with DNA and histones participating in the modification of chromatin structure important for gene expression regulation.
Biological function summary

L3MBTL1 functions as a transcriptional repressor involved in cell cycle regulation and differentiation. It acts by binding to methylated lysines on histone tails affecting chromatin compaction and accessibility. L3MBTL1 is part of the Polycomb group (PcG) protein complex which maintains genes in a repressed state ensuring proper cell fate determination.

Pathways

L3MBTL1 is an important component in chromatin modification and transcriptional repression pathways. It participates in pathways such as the Polycomb Repressive Complex 1 (PRC1)-mediated gene silencing pathway. L3MBTL1 interacts with other Polycomb proteins such as BMI1 and RING1 to ensure effective repression of target genes.

L3MBTL1 has a strong connection to certain types of cancer and genetic syndromes. Alterations in L3MBTL1 expression or function can contribute to hematological malignancies like acute myeloid leukemia. It is also linked to developmental disorders due to its role in epigenetic regulation during development. L3MBTL1 may interact with proteins like EZH2 a related PcG protein influencing the progression of these diseases.
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Specifications

Form

Liquid

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General info

Function

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes : acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.

Post-translational modifications

Ubiquitinated in a VCP/p97-dependent way following DNA damage, leading to its removal from DNA damage sites, promoting accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited to DNA damage sites.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes : acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
See full target information L3MBTL1
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