Recombinant human Lactate Dehydrogenase B/LDH-B protein is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A T L K E K L I A P V A E E E A T V P N N K I T V V G V G Q V G M A C A I S I L G K S L A D E L A L V D V L E D K L K G E M M D L Q H G S L F L Q T P K I V A D K D Y S V T A N S K I V V V T A G V R Q Q E G E S R L N L V Q R N V N V F K F I I P Q I V K Y S P D C I I I V V S N P V D I L T Y V T W K L S G L P K H R V I G S G C N L D S A R F R Y L M A E K L G I H P S S C H G W I L G E H G D S S V A V W S G V N V A G V S L Q E L N P E M G T D N D S E N W K E V H K M V V E S A Y E V I K L K G Y T N W A I G L S V A D L I E S M L K N L S R I H P V S T M V K G M Y G I E N E V F L S L P C I L N A R G L T S V I N Q K L K D D E V A Q L K K S A D T L W D I Q K D L K D L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB
Recommended products
Recombinant human Lactate Dehydrogenase B/LDH-B protein is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Activity Assay
- Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37°C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
- Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
- Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
- Mix by inversion and record the increase at A340nm for 5 minutes.
- Accession
- P07195-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A T L K E K L I A P V A E E E A T V P N N K I T V V G V G Q V G M A C A I S I L G K S L A D E L A L V D V L E D K L K G E M M D L Q H G S L F L Q T P K I V A D K D Y S V T A N S K I V V V T A G V R Q Q E G E S R L N L V Q R N V N V F K F I I P Q I V K Y S P D C I I I V V S N P V D I L T Y V T W K L S G L P K H R V I G S G C N L D S A R F R Y L M A E K L G I H P S S C H G W I L G E H G D S S V A V W S G V N V A G V S L Q E L N P E M G T D N D S E N W K E V H K M V V E S A Y E V I K L K G Y T N W A I G L S V A D L I E S M L K N L S R I H P V S T M V K G M Y G I E N E V F L S L P C I L N A R G L T S V I N Q K L K D D E V A Q L K K S A D T L W D I Q K D L K D L
- Accession
- P07195
- Protein length
- Full Length
- Predicted molecular weight
- 36.6 kDa
- Amino acids
- 1 to 334
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab96765 is purified by using conventional chromatography techniques.
General info
- Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
- Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as Lactate Dehydrogenase B.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.
- Biological function summary
LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.
- Pathways
Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.
- Associated diseases and disorders
Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.
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1 product image
SDS-PAGE - Recombinant human Lactate Dehydrogenase B/LDH-B protein (ab96765)
15% SDS-PAGE showing ab96765 at approximately 38.8kDa (3μg).
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