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AB96765

Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus)

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Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

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SDS-PAGE - Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus) (AB96765)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Lactate Dehydrogenase B/LDH-B protein (His tag N-Terminus) (AB96765)

15% SDS-PAGE showing ab96765 at approximately 38.8kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

Activity Assay

  1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37°C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
  2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
  3. Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
  4. Mix by inversion and record the increase at A340nm for 5 minutes.
Accession

P07195

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Lactate Dehydrogenase B.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL","proteinLength":"Full Length","predictedMolecularWeight":"36.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07195","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.
Biological function summary

LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.

Pathways

Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.

Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.
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Specifications

Form

Liquid

Additional notes

ab96765 is purified by using conventional chromatography techniques.

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General info

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
See full target information LDHB
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