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AB93699

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus)

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(5 Publications)

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

2 Images
Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)
  • WB

Unknown

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody (<a href='/en-us/products/primary-antibodies/lactate-dehydrogenase-antibody-ab47010'>ab47010</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (ab93699) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (AB93699)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (AB93699)

SDS-PAGE analysis of Recombinant Human Lactate Dehydrogenase protein (ab93699), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human Lactate Dehydrogenase protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

Activity Assay

  1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
  2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.
  3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
  4. Mix by inversion and record the increase at A340nm for 5 minutes.
Accession

P00338

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab93699 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/lactate-dehydrogenase-antibody-ab47010'>ab47010</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Concentration determined by Bradford assay.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF","proteinLength":"Full Length","predictedMolecularWeight":"38.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":332,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00338","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.
Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.
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Specifications

Form

Liquid

Additional notes

ab93699 is purified by using conventional chromatography techniques.

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General info

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Post-translational modifications

ISGylated.

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Product protocols

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
See full target information LDHA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:64 PubMed38233415

2024

FKBP10 promotes clear cell renal cell carcinoma progression and regulates sensitivity to the HIF2α blockade by facilitating LDHA phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Liu,Zhihao Zou,Lingwu Chen,Yuanfa Feng,Jianheng Ye,Yulin Deng,Xuejin Zhu,Yixun Zhang,Jundong Lin,Shanghua Cai,Zhenfeng Tang,Yingke Liang,Jianming Lu,Yangjia Zhuo,Zhaodong Han,Xiaohui Ling,Yuxiang Liang,Zongren Wang,Weide Zhong

ACS sensors 6:842-851 PubMed33724791

2021

Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman Russom

Molecules (Basel, Switzerland) 25: PubMed32121469

2020

Chiral Pyridine-3,5-bis- (L-phenylalaninyl-L-leucinyl) Schiff Base Peptides as Potential Anticancer Agents: Design, Synthesis, and Molecular Docking Studies Targeting Lactate Dehydrogenase-A.

Applications

Unspecified application

Species

Unspecified reactive species

Abd El-Galil E Amr,Randa E Abdel Mageid,Mohamed El-Naggar,Ahmed M Naglah,Eman S Nossier,Elsayed A Elsayed

Molecular & cellular proteomics : MCP 18:1330-1344 PubMed31010818

2019

Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Tommy Weiss-Sadan,Gal Itzhak,Farnusch Kaschani,Zhanru Yu,Mohamed Mahameed,Adi Anaki,Yael Ben-Nun,Emmanuelle Merquiol,Boaz Tirosh,Benedikt Kessler,Markus Kaiser,Galia Blum

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson
View all publications
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