Recombinant human Lactate Dehydrogenase protein (Active) is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
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- ACS sensors 6:842-8512021Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman RussomPubMed 33724791
- Molecules (Basel, Switzerland) 25:2020Chiral Pyridine-3,5-bis- (L-phenylalaninyl-L-leucinyl) Schiff Base Peptides as Potential Anticancer Agents: Design, Synthesis, and Molecular Docking Studies Targeting Lactate Dehydrogenase-A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbd El-Galil E Amr et. al.PubMed 32121469
- Molecular & cellular proteomics : MCP 18:1330-13442019Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTommy Weiss-Sadan et. al.PubMed 31010818
- Nature chemical biology 13:494-5002017L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew M Intlekofer et. al.PubMed 28263965
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A T L K D Q L I Y N L L K E E Q T P Q N K I T V V G V G A V G M A C A I S I L M K D L A D E L A L V D V I E D K L K G E M M D L Q H G S L F L R T P K I V S G K D Y N V T A N S K L V I I T A G A R Q Q E G E S R L N L V Q R N V N I F K F I I P N V V K Y S P N C K L L I V S N P V D I L T Y V A W K I S G F P K N R V I G S G C N L D S A R F R Y L M G E R L G V H P L S C H G W V L G E H G D S S V P V W S G M N V A G V S L K T L H P D L G T D K D K E Q W K E V H K Q V V E S A Y E V I K L K G Y T S W A I G L S V A D L A E S I M K N L R R V H P V S T M I K G L Y G I K D D V F L S V P C I L G Q N G I S D L V K V T L T S E E E A R L K K S A D T L W G I Q K E L Q F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab93699 can be used as a WB positive control in conjunction with Anti-Lactate Dehydrogenase antibody ab47010. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M
Recommended products
Recombinant human Lactate Dehydrogenase protein (Active) is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Activity Assay
- Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
- Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.
- Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
- Mix by inversion and record the increase at A340nm for 5 minutes.
- Accession
- P00338-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A T L K D Q L I Y N L L K E E Q T P Q N K I T V V G V G A V G M A C A I S I L M K D L A D E L A L V D V I E D K L K G E M M D L Q H G S L F L R T P K I V S G K D Y N V T A N S K L V I I T A G A R Q Q E G E S R L N L V Q R N V N I F K F I I P N V V K Y S P N C K L L I V S N P V D I L T Y V A W K I S G F P K N R V I G S G C N L D S A R F R Y L M G E R L G V H P L S C H G W V L G E H G D S S V P V W S G M N V A G V S L K T L H P D L G T D K D K E Q W K E V H K Q V V E S A Y E V I K L K G Y T S W A I G L S V A D L A E S I M K N L R R V H P V S T M I K G L Y G I K D D V F L S V P C I L G Q N G I S D L V K V T L T S E E E A R L K K S A D T L W G I Q K E L Q F
- Accession
- P00338
- Protein length
- Full Length
- Predicted molecular weight
- 38.8 kDa
- Amino acids
- 1 to 332
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab93699 is purified by using conventional chromatography techniques.
General info
- Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
- Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
- Post-translational modifications
ISGylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Concentration determined by Bradford assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.
- Biological function summary
Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.
- Pathways
LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.
- Associated diseases and disorders
Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.
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2 product images
SDS-PAGE - Recombinant human Lactate Dehydrogenase protein (Active) (ab93699)
SDS-PAGE analysis of Recombinant Human Lactate Dehydrogenase protein (ab93699), under reducing conditions. Proteins visualized by coomassie blue stain.
Lane 1: Molecular Weight Standards
Lane 2: 3 μg Recombinant Human Lactate Dehydrogenase protein
Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (ab93699)
All lanes: Western blot - Anti-Lactate Dehydrogenase antibody (Anti-Lactate Dehydrogenase antibody ab47010) at 1 µg/mL
All lanes: Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (ab93699) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Exposure time: 10s
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