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Recombinant Human LAG-3 protein (Biotin) is a Human Full Length protein, in the 23 to 450 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human LAG-3 protein (Biotin) (AB198441), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Tags

Avi tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Conjugation

Biotin

Amino acid sequence

L Q P G A E V P V V W A Q E G A P A Q L P C S P T I P L Q D L S L L R R A G V T W Q H Q P D S G P P A A A P G H P L A P G P H P A A P S S W G P R P R R Y T V L S V G P G G L R S G R L P L Q P R V Q L D E R G R Q R G D F S L W L R P A R R A D A G E Y R A A V H L R D R A L S C R L R L R L G Q A S M T A S P P G S L R A S D W V I L N C S F S R P D R P A S V H W F R N R G Q G R V P V R E S P H H H L A E S F L F L P Q V S P M D S G P W G C I L T Y R D G F N V S I M Y N L T V L G L E P P T P L T V Y A G A G S R V G L P C R L P A G V G T R S F L T A K W T P P G G G P D L L V T G D N G D F T L R L E D V S Q A Q A G T Y T C H I H L Q E Q Q L N A T V T L A I I T V T P K S F G S P G S L G K L L C E V T P V S G Q E R F V W S S L D T P S Q R S F S G P W L E A Q E A Q L L S Q P W Q C Q L Y Q G E R L L G A A V Y F T E L S S P G A Q R S G R A P G A L P A G H L I E I E G R M D P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K G G G L N D I F E A Q K I E W H E

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185).Secreted lymphocyte activation gene 3 proteinMay function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

Alternative names

Recommended products

Recombinant Human LAG-3 protein (Biotin) is a Human Full Length protein, in the 23 to 450 aa range, expressed in HEK 293, with >90% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE

Conjugation

Biotin

Accession
P18627-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Sequence info

Amino acid sequence

L Q P G A E V P V V W A Q E G A P A Q L P C S P T I P L Q D L S L L R R A G V T W Q H Q P D S G P P A A A P G H P L A P G P H P A A P S S W G P R P R R Y T V L S V G P G G L R S G R L P L Q P R V Q L D E R G R Q R G D F S L W L R P A R R A D A G E Y R A A V H L R D R A L S C R L R L R L G Q A S M T A S P P G S L R A S D W V I L N C S F S R P D R P A S V H W F R N R G Q G R V P V R E S P H H H L A E S F L F L P Q V S P M D S G P W G C I L T Y R D G F N V S I M Y N L T V L G L E P P T P L T V Y A G A G S R V G L P C R L P A G V G T R S F L T A K W T P P G G G P D L L V T G D N G D F T L R L E D V S Q A Q A G T Y T C H I H L Q E Q Q L N A T V T L A I I T V T P K S F G S P G S L G K L L C E V T P V S G Q E R F V W S S L D T P S Q R S F S G P W L E A Q E A Q L L S Q P W Q C Q L Y Q G E R L L G A A V Y F T E L S S P G A Q R S G R A P G A L P A G H L I E I E G R M D P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K G G G L N D I F E A Q K I E W H E

Accession

P18627

Protein length

Full Length

Predicted molecular weight

74.8 kDa

Amino acids

23 to 450

Nature

Recombinant

Tags

Avi tag C-Terminus

Specifications

Form

Liquid

General info

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185).

Sequence similarities

Belongs to the LAG3 family.

Post-translational modifications

Lymphocyte activation gene 3 protein

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This product was previously labelled as Lymphocyte Activation Gene 3.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

Associated diseases and disorders

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

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1 product image

  • SDS-PAGE - Recombinant Human LAG-3 protein (Biotin) (ab198441), expandable thumbnail

    SDS-PAGE - Recombinant Human LAG-3 protein (Biotin) (ab198441)

    4-20% SDS-PAGE analysis of 2.8 μg ab198441 with Coomassie staining.

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Product protocols

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