Recombinant Human LAMA3 protein is a Human Fragment protein, in the 29 to 138 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
S S Q Q Q R V P F L Q P P G Q S Q L Q A S Y V E F R P S Q G C S P G Y Y R D H K G L Y T G R C V P C N C N G H S N Q C Q D G S G I C V N C Q H N T A G E H C E R C Q E G Y Y G N A V H G S C R A C P C P H T N S F A T G C V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Alternative names
LAMNA, LAMA3, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170
Recommended products
Recombinant Human LAMA3 protein is a Human Fragment protein, in the 29 to 138 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q16787-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- S S Q Q Q R V P F L Q P P G Q S Q L Q A S Y V E F R P S Q G C S P G Y Y R D H K G L Y T G R C V P C N C N G H S N Q C Q D G S G I C V N C Q H N T A G E H C E R C Q E G Y Y G N A V H G S C R A C P C P H T N S F A T G C V
- Accession
- Q16787
- Protein length
- Fragment
- Amino acids
- 29 to 138
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.
- Biological function summary
LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.
- Pathways
Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.
- Associated diseases and disorders
Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.
Product promise
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1 product image
SDS-PAGE - Recombinant Human LAMA3 protein (ab158810)
ab158810 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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Product protocols
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