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Recombinant Human LAMC2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human LAMC2 Protein Standard (His tag) (AB316641), expandable thumbnail
  • Sandwich ELISA - Recombinant Human LAMC2 Protein Standard (His tag) (AB316641), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

Biologically active

No

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

Alternative names

Recombinant Human LAMC2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Applications

sELISA, SDS-PAGE

Accession
Q13753-1
Animal free

Yes

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

Q13753

Protein length

Fragment

Predicted molecular weight

65.3 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

Post-translational modifications

O-glycosylated; contains chondroitin sulfate (CS). CS attachment is on either Ser-803 or Ser-805.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The LAMC2 protein also known as laminin subunit gamma-2 is an essential component of the extracellular matrix. It is a subunit of the laminin-332 complex formerly referred to as laminin-5 and has an approximate mass of 160 kDa. LAMC2 is widely expressed in various tissues most prominently in epithelial tissues. It localizes at the basement membrane zone where epithelial and mesenchymal tissues meet.

Biological function summary

LAMC2 plays a critical role in cell adhesion migration and signal transduction. Laminin-332 which includes LAMC2 binds to integrin receptors on cell surfaces facilitating cell-matrix interactions and communication. This complex is important for basement membrane stability and tissue organization and it is actively involved in wound healing by promoting keratinocyte migration and melanin transport.

Pathways

LAMC2 integrates into several important extracellular matrix pathways including the integrin signaling pathway. These pathways involve interactions with proteins like integrin alpha-6/beta-4 which are linked to cellular adhesion and mobility. LAMC2 also contributes to epithelial cell functions and is involved in the PI3K/AKT signaling pathway highlighting its importance in cell proliferation and survival.

Associated diseases and disorders

LAMC2 has significant associations with conditions like dystrophic epidermolysis bullosa and invasive carcinomas. In dystrophic epidermolysis bullosa mutations in LAMC2 disrupt the integrity of the basement membrane leading to fragile skin and blistering. In cancer abnormal expression or alteration of LAMC2 can promote tumor invasion and metastasis often in conjunction with overexpression of matrix metalloproteinases (MMPs) that degrade the extracellular matrix therefore facilitating cancer cell infiltration.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • SDS-PAGE - Recombinant Human LAMC2 Protein Standard (His tag) (ab316641), expandable thumbnail

    SDS-PAGE - Recombinant Human LAMC2 Protein Standard (His tag) (ab316641)

    SDS-PAGE analysis of ab316641 under reducing conditions for 2ug protein.

  • Sandwich ELISA - Recombinant Human LAMC2 Protein Standard (His tag) (ab316641), expandable thumbnail

    Sandwich ELISA - Recombinant Human LAMC2 Protein Standard (His tag) (ab316641)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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