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AB235807

Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged)

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Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) is a Human Fragment protein, in the 3401 to 3692 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

3 Images
Mass Spectrometry - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab235807 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) Laminin alpha 5/LAMA5.

Mass Spectrometry - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab235807 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) Laminin alpha 5/LAMA5.

SDS-PAGE - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Laminin alpha 5/LAMA5 protein (Tagged) (AB235807)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel analysis of ab235807.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O15230

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Laminin alpha 5
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Sequence info

[{"sequence":"FVAQMEGLGTRLRAQSRQRSRPGRWHKVSVRWEKNRILLVTDGARAWSQEGPHRQHQGAEHPQPHTLFVGGLPASSHSSKLPVTVGFSGCVKRLRLHGRPLGAPTRMAGVTPCILGPLEAGLFFPGSGGVITLDLPGATLPDVGLELEVRPLAVTGLIFHLGQARTPPYLQLQVTEKQVLLRADDGAGEFSTSVTRPSVLCDGQWHRLAVMKSGNVLRLEVDAQSNHTVGPLLAAAAGAPAPLYLGGLPEPMAVQPWPPAYCGCMRRLAVNRSPVAMTRSVEVHGAVGASGC","proteinLength":"Fragment","predictedMolecularWeight":"47.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":3692,"aminoAcidStart":3401,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15230","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.
Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.
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Specifications

Form

Liquid

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General info

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

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Product protocols

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).
See full target information LAMA5
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